| Texto completo | |
| Autor(es): |
de Lucas, Rosymar Coutinho
[1, 2]
;
de Oliveira, Tassio Brito
[1]
;
Lima, Matheus Sanita
[3]
;
Pasin, Thiago Machado
[2]
;
de Almeida Scarcella, Ana Silvia
[2]
;
Prade, Rolf Alexander
[4]
;
Segato, Fernando
[5]
;
de Moraes Polizeli, Maria de Lourdes Teixeira
[1, 2]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Bioquim & Imunol, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil
[3] Univ Western Ontario, Dept Biol, London, ON - Canada
[4] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 - USA
[5] Univ Sao Paulo, Escola Engn Lorena, Dept Biotecnol, Lorena, SP - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOMASS CONVERSION AND BIOREFINERY; v. 12, n. 2, p. 491-499, FEB 2022. |
| Citações Web of Science: | 5 |
| Resumo | |
The polysaccharides in the primary plant cell wall are a renewable energy source for biofuel production. However, these polysaccharides are not readily available for bioconversion, and large enzyme sets are required to deconstruct them. Here, we aimed to improve the glucan conversion using recombinant hemicellulases and esterase as a treatment in exploded and sugarcane bagasses (SCB), followed by the addition of commercial CBH I to prevent its inhibition by hemicellulases products. A high secretion level of the recombinant enzymes was observed on SDS-PAGE. The highest activities were verified at a temperature and pH ranging from 40 to 55 degrees C and 4.5 to 6.0, respectively. The released reducing sugar analysis showed that all enzymes act better on SCB, with xylanase C (XynC) presenting the best activity (0.54 U/mg of protein). The high-performance liquid chromatography (HPLC) analysis demonstrated that 24 h of pretreatment was enough to reach maximum glucan conversion. The best synergy was achieved between XynC and CBH I on SCB, 1.4%. All results showed that the enzymes acted better on SCB, which can be related to the biomass composition and its molecular structure. The enzymatic pretreatment of SCB with XynC was essential to improve the glucan conversion by CBH I. (AU) | |
| Processo FAPESP: | 17/09000-4 - Efeitos da temperatura e da deficiência hídrica, em cenário climático futuro, na comunidade de fungos em solo tropical e na seleção de linhagens produtoras de óxido nitroso |
| Beneficiário: | Tássio Brito de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |