| Texto completo | |
| Autor(es): |
Salesa, Beatriz
[1]
;
Tunon-Molina, Alberto
[1]
;
Cano-Vicent, Alba
[1]
;
Assis, Marcelo
[2]
;
Andres, Juan
[2]
;
Serrano-Aroca, Angel
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Catolica Valencia San Vicente Martir, Ctr Invest Traslac San Alberto Magno, Biomat & Bioengn Lab, C-Guillem Castro 94, Valencia 46001 - Spain
[2] Univ Jaume, Dept Phys & Analyt Chem, Castellon de La Plana 12071 - Spain
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | APPLIED SCIENCES-BASEL; v. 12, n. 2 JAN 2022. |
| Citações Web of Science: | 1 |
| Resumo | |
Multi-layer graphene (2-10 layers), also called graphene nanoplatelets (GNPs), is a carbon-based nanomaterial (CBN) type with excellent properties desirable for many biomedical applications. Despite the promising advantages reported of GNPs, nanoscale materials may also present a potential hazard to humans. Therefore, in this study, the in vivo toxicity of these nanomaterials at a wide range of concentrations from 12.5 to 500 mu g/mL was evaluated in the Caenorhabditis elegans model for 24 h (acute toxicity) and 72 h (chronic toxicity). Furthermore, their in vitro toxicity (from 0 to 10 mu g/mL for 12 and 24 h), proliferative activity at 72 and 96 h, and their effect on the expression of thirteen genes in human keratinocytes HaCaT cells were studied. The physico-chemical and morphological aspects of the GNPs used in this study were analyzed by Raman scattering spectroscopy, electron microscopy, zeta potential as a function of pH, and particle size measurements by dynamic light scattering. The results of this study showed that GNPs showed in vivo non-toxic concentrations of 25 and 12.5 mu g/mL for 24 h, and at 12.5 mu g/mL for 72 h. Moreover, GNPs present time-dependent cytotoxicity (EC50 of 1.142 mu g/mL and 0.760 mu g/mL at 12 h and 24 h, respectively) and significant proliferative activity at the non-toxic concentrations of 0.005 and 0.01 mu g/mL in the HaCaT cell line. The gene expression study showed that this multi-layer-graphene is capable of up-regulating six of the thirteen genes of human keratinocytes (SOD1, CAT, TGFB1, FN1, CDH1, and FBN), two more genes than other CBNs in their oxidized form such as multi-layer graphene oxide. Therefore, all these results reinforce the promising use of these CBNs in biomedical fields such as wound healing and skin tissue engineering. (AU) | |
| Processo FAPESP: | 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais |
| Beneficiário: | Elson Longo da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |