econstruction of regulatory network predicts transcription factors driving the dynamics of zebrafish heart regeneratio

Autor(es)

Nunes, Leandro Silva ^[1] ; Domingues, William Borges ^[1] ; Kremer, Frederico Schmitt ^[2] ; Pinhal, Danillo ^[3] ; Campos, Vinicius Farias ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Pelotas, Ctr Desenvolvimento Tecnol, Lab Genom Estrutural, Programa Posgrad Biotecnol, Pelotas, RS - Brazil

^[2] Univ Fed Pelotas, Ctr Desenvolvimento Tecnol, Lab Bioinformat & Proteom, Programa Posgrad Biotecnol, Pelotas, RS - Brazil

^[3] Univ Estadual Paulista UNESP, Inst Biociencias Botucatu, Dept Genet, Lab Genom & Evolucao Mol, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Gene; v. 819, APR 20 2022.
• Citações Web of Science: 0

Resumo

The limited regenerative capacity in mammals has serious implications for cardiac tissue damage. Meanwhile, zebrafish has a high regenerative capacity, but the regulation of the heart healing process has yet to be eluci-dated. The dynamic nature of cardiac regeneration requires consideration of the inherent temporal dimension of this process. Here, we conducted a systematic review to find genes that define the regenerative cell state of the zebrafish heart. We then performed an in silico temporal gene regulatory network analysis using transcriptomic data from the zebrafish heart regenerative process obtained from databases. In this analysis, the genes found in the systematic review were used to represent the final cell state of the transition process from a non-regenerative cell state to a regenerative state. We found 135 transcription factors driving the cellular state transition process during zebrafish cardiac regeneration, including Hand2, Nkx2.5, Tbx20, Fosl1, Fosb, Junb, Vdr, Wt1, and Tcf21 previously reported for playing a key role in tissue regeneration. Furthermore, we demonstrate that most reg-ulators are activated in the first days post-injury, indicating that the transition from a non-regenerative to a regenerative state occurs promptly. (AU)

• Processo FAPESP: 18/05484-0 - Análise funcional de microRNAs na desdiferenciação celular durante o processo de regeneração tecidual no modelo zebrafish
• Beneficiário: Danillo Pinhal
• Modalidade de apoio: Auxílio à Pesquisa - Regular