mino Acids and IGF1 Regulation of Fish Muscle Growth Revealed by Transcriptome and microRNAome Integrative Analyses of Pacu (Piaractus mesopotamicus) Myotube

Autor(es)

Duran, Bruno Oliveira Silva ^[1] ; Zanella, Bruna Tereza Thomazini ^[2] ; Perez, Erika Stefani ^[2] ; Mareco, Edson Assuncao ^[3] ; Blasco, Josefina ^[4] ; Dal-Pai-Silva, Maeli ^[2] ; Garcia de la Serrana, Daniel ^[4]

Afiliação do(s) autor(es)

^[1] Fed Univ Goias UFG, Dept Histol Embryol & Cell Biol, Inst Biol Sci, BR-74690900 Goiania, Go - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Struct & Funct Biol, Inst Biosci, BR-18618689 Botucatu, SP - Brazil

^[3] Univ Western Sao Paulo UNOESTE, Environm & Reg Dev Grad Program, BR-19050680 Presidente Prudente, SP - Brazil

^[4] Univ Barcelona, Fac Biol, Dept Cell Biol Physiol & Immunol, Barcelona 08028 - Spain

• Tipo de documento: Artigo Científico
• Fonte: NTERNATIONAL JOURNAL OF MOLECULAR SCIENCE; v. 23, n. 3 FEB 2022.
• Citações Web of Science: 0

Resumo

Amino acids (AA) and IGF1 have been demonstrated to play essential roles in protein synthesis and fish muscle growth. The myoblast cell culture is useful for studying muscle regulation, and omics data have contributed enormously to understanding its molecular biology. However, to our knowledge, no study has performed the large-scale sequencing of fish-cultured muscle cells stimulated with pro-growth signals. In this work, we obtained the transcriptome and microRNAome of pacu (Piaractus mesopotamicus)-cultured myotubes treated with AA or IGF1. We identified 1228 and 534 genes differentially expressed by AA and IGF1. An enrichment analysis showed that AA treatment induced chromosomal changes, mitosis, and muscle differentiation, while IGF1 modulated IGF/PI3K signaling, metabolic alteration, and matrix structure. In addition, potential molecular markers were similarly modulated by both treatments. Muscle-miRNAs (miR-1, -133, -206 and -499) were up-regulated, especially in AA samples, and we identified molecular networks with omics integration. Two pairs of genes and miRNAs demonstrated a high-level relationship, and involvement in myogenesis and muscle growth: marcksb and miR-29b in AA, and mmp14b and miR-338-5p in IGF1. Our work helps to elucidate fish muscle physiology and metabolism, highlights potential molecular markers, and creates a perspective for improvements in aquaculture and in in vitro meat production. (AU)

• Processo FAPESP: 18/24575-6 - Análises integrativas e comparativas do fosfoproteoma de culturas celulares de mioblastos do pacu (Piaractus mesopotamicus) e gilthead seabream (Sparus aurata) submetidas a tratamentos com fatores de crescimento.
• Beneficiário: Bruno Oliveira da Silva Duran
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/26428-0 - Compreensão das interações entre o fosfoproteoma, transcriptoma e microRNAoma em resposta a sinais pró-crescimento em miotubos de pacu (Piaractus mesopotamicus) e gilthead sea bream (Sparus aurata).
• Beneficiário: Maeli Dal Pai
• Modalidade de apoio: Auxílio à Pesquisa - Regular