Lysozyme activity as an indicator of innate immunity of tilapia (Oreochromis niloticus) when challenged with LPS and Streptococcus agalactiae

Autor(es)

Jaqueline Dalbello Biller ^[1] ; Gustavo do Valle Polycarpo ^[2] ; Basia Schlichting Moromizato ^[3] ; Ana Paula Diogo Sidekerskis ^[4] ; Thaís Daltoso da Silva ^[5] ; Ingrid Camargo dos Reis ^[6] ; Camino Fierro-Castro ^[7]

Afiliação do(s) autor(es)

^[1] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Agrárias e Tecnológicas - Brasil

^[2] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Agrárias e Tecnológicas - Brasil

^[3] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Agrárias e Tecnológicas - Brasil

^[4] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Agrárias e Tecnológicas - Brasil

^[5] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Programa de Pós-Graduação em Aquicultura - Brasil

^[6] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Programa de Pós-Graduação em Ciência e Tecnologia Animal - Brasil

^[7] Universidad de León. Facultad de Ciencias Biológicas y Ambientales. Departamento de Biología Molecular

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE; v. 50, 2021-11-29.

Resumo

ABSTRACT The objective of this study was to fully describe the protocol with standardized modifications and evaluate the lysozyme activity, an indicator of innate immunity in tilapia, to compare lipopolysaccharide (LPS) with Streptococcus agalactiae injections. Lysozyme was determined in serum using the turbidimetric method, in which lysozyme activity was evaluated by Micrococcus lysodeikticus lysis, with modifications for microplate assay. The experiment was conducted in a completely randomized design. Juvenile tilapia was divided in the following six treatments: challenged with phosphate buffer PBS (control) and 100, 250, 500, and 600 μg kg−1 LPS and S. agalactiae. All treatments were challenged for 72 h and seven days and then sampled to determine lysozyme activity. After 72 h or seven days, concentrations of LPS promoted changes in lysozyme production, either lesser or equal, depending on concentration when compared with fish injected with S. agalactiae. It was possible to standardize the analysis and determine that the treatment with LPS promotes immunomodulation at a concentration of 250 μg kg−1 LPS, this response being similar to challenge with S. agalactiae. (AU)

• Processo FAPESP: 12/22016-3 - Estudo do potencial imunoestimulante do b-glucano e seu modo de ação visando novos protocolos de administração em tilápia, Oreochromis niloticus
• Beneficiário: Jaqueline Dalbello Biller Takahashi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores