Differentially expressed plasmatic microRNAs in Brazilian patients with Coronavirus disease 2019 (COVID-19): preliminary results

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Nicoletti, Aline de Souza ; Visacri, Marilia Berlofa ; da Silva Correa da Ronda, Carla Regina ; do Nascimento Silva Vasconcelos, Pedro Eduardo ; Franca Quintanilha, Julia Coelho ; de Souza, Rafael Nogueira ; Ventura, Deise de Souza ; Eguti, Adriana ; de Souza Silva, Lilian Ferreira ; Perroud Junior, Mauricio Wesley ; Catharino, Rodrigo Ramos ; Reis, Leonardo Oliveira ; dos Santos, Luiz Augusto ; Duran, Nelson ; Favaro, Wagner Jose ; Lancellotti, Marcelo ; da Costa, Jose Luiz ; Moriel, Patricia ; Pincinato, Eder de Carvalho

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY REPORTS; v. N/A, p. 13-pg., 2022-03-17.

Resumo

Background Coronavirus disease 2019 (COVID-19) is caused by a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It is known that host microRNAs (miRNAs) can be modulated to favor viral infection or to protect the host. Herein, we report preliminary results of a study aiming at identifying differentially expressed plasmatic miRNAs in Brazilian patients with COVID-19. Methods and results miRNAs were extracted from the plasma of eight patients with COVID-19 (four patients with mild COVID-19 and four patients with severe/critical COVID-19) and four healthy controls. Patients and controls were matched for sex and age. miRNA expression levels were detected using high-throughput sequencing. Differential miRNA expression and enrichment analyses were further evaluated. A total of 18 miRNAs were differentially expressed between patients with COVID-19 and controls. miR-4433b-5p, miR-6780b-3p, miR-6883-3p, miR-320b, miR-7111-3p, miR-4755-3p, miR-320c, and miR-6511a-3p were the most important miRNAs significantly involved in the PI3K/AKT, Wnt/beta-catenin, and STAT3 signaling pathways. Moreover, 42 miRNAs were differentially expressed between severe/critical and mild patients with COVID-19. miR-451a, miR-101-3p, miR-185-5p, miR-30d-5p, miR-25-3p, miR-342-3p, miR-30e-5p, miR-150-5p, miR-15b-5p, and miR-29c-3p were the most important miRNAs significantly involved in the Wnt/beta-catenin, NF-kappa beta, and STAT3 signaling pathways. Conclusions If validated by quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in a larger number of participants, the miRNAs identified in this study might be used as possible biomarkers for the diagnosis and severity of COVID-19. (AU)

• Processo FAPESP: 21/04669-9 - MiRNAs e polimorfismos no receptor da enzima conversora de angiotensina 2 como possíveis biomarcadores de doença induzida elo SARS-CoV-2
• Beneficiário: Eder de Carvalho Pincinato
• Modalidade de apoio: Auxílio à Pesquisa - Regular