Optimizing studies of phagocytic activity by flowsight cytometry in amphibians

Autor(es)

Monteiro Titon, Stefanny Christie ; Assis, Vania Regina ; Titon Junior, Braz ; Kinker, Gabriela Sarti ; Teixeira de Queiroz Hazarbassanov, Nicolle Gilda ; Lima, Alan Siqueira ; Massoco, Cristina de Oliveira ; Fernandes, Pedro A. ; Gomes, Fernando Ribeiro ; Markus, Regina P.

• Tipo de documento: Artigo Científico
• Fonte: South American Journal of Herpetology; v. 23, n. 1, p. 9-pg., 2022-04-01.

Resumo

Phagocytosis is a primary and highly conserved mechanism for clearing the extracellular milieu from pathogens and debris. In amphibians, the lack of antibodies for characterizing the different phenotypes of phagocytic cells has impaired the study of the phagocytic process. We used conventional and flowsight cytometry to determine immune cells' phagocytic activity from the blood and peritoneum of toads by in vitro and in vivo assays. Macrophage-like and neutrophil-like cells were clustered and analyzed according to cell morphology and the number of internalized zymosan particles by flowsight cytometry. We identified peritoneal and blood phagocytes (macrophage-like/monocyte-like and neutrophil-like) and lymphocyte-like cells. Besides, we observed monocyte-like/macrophage-like and neutrophil-like cells engulfing up to seven zymosan particles. Assessing the phagocytic activity from blood and peritoneal phagocytes using in vitro and in vivo assays brings better insights into phagocytosis in amphibian immune cells from distinct body compartments and approaches. Moreover, it is worth highlighting the importance of morphologically identifying the cells and evaluating the number of internalized particles by flowsight cytometry, a valuable asset to further explore phagocytosis and other cellular processes in amphibians under field and laboratory conditions. (AU)

• Processo FAPESP: 13/00900-1 - Estudo comparativo das relações entre imunocompetência e níveis plasmáticos de esteróides inter-renais e gonadais em sapos
• Beneficiário: Fernando Ribeiro Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/16320-7 - Impactos das mudanças climáticas e ambientais sobre a fauna: uma abordagem integrativa
• Beneficiário: Carlos Arturo Navas Iannini
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático
• Processo FAPESP: 13/13691-1 - Eixo imune-pineal: integrando a biologia do tempo em condições fisiológicas, fisiopatológicas e patológicas
• Beneficiário: Regina Pekelmann Markus
• Modalidade de apoio: Auxílio à Pesquisa - Temático