Host cell interactions of novel antigenic membrane proteins of Mycoplasma agalactiae

Autor(es)

Barbosa, Maysa Santos ; Marques, Lucas Miranda ; Timenetsky, Jorge ; Rosengarten, Renate ; Spergser, Joachim ; Chopra-Dewasthaly, Rohini

• Tipo de documento: Artigo Científico
• Fonte: BMC Microbiology; v. 22, n. 1, p. 13-pg., 2022-04-08.

Resumo

Background Mycoplasma agalactiae is the main etiological agent of Contagious Agalactia syndrome of small ruminants notifiable to the World Organization for Animal Health. Despite serious economic losses, successful vaccines are unavailable, largely because its colonization and invasion factors are not well understood. This study evaluates the role of two recently identified antigenic proteins (MAG_1560, MAG_6130) and the cytadhesin P40 in pathogenicity related phenotypes. Results Adhesion to HeLa and sheep primary mammary stromal cells (MSC) was evaluated using ELISA, as well as in vitro adhesion assays on monolayer cell cultures. The results demonstrated MAG_6130 as a novel adhesin of M. agalactiae whose capacity to adhere to eukaryotic cells was significantly reduced by specific antiserum. Additionally, these proteins exhibited significant binding to plasminogen and extracellular matrix (ECM) proteins like lactoferrin, fibrinogen and fibronectin, a feature that could potentially support the pathogen in host colonization, tissue migration and immune evasion. Furthermore, these proteins played a detrimental role on the host cell proliferation and viability and were observed to activate pro-apoptotic genes indicating their involvement in cell death when eukaryotic cells were infected with M. agalactiae. Conclusions To summarize, the hypothetical protein corresponding to MAG_6130 has not only been assigned novel adhesion functions but together with P40 it is demonstrated for the first time to bind to lactoferrin and ECM proteins thereby playing important roles in host colonization and pathogenicity. (AU)

• Processo FAPESP: 17/25686-3 - Desenvolvimento de teste de diagnóstico e seleção de candidatos vacinais para micoplasmose em bovinos e caprinos
• Beneficiário: Jorge Timenetsky
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/03425-9 - Caracterização antigênica de proteínas de membrana de Mycoplasma agalactiae e suas funções na virulência e patogenicidade in vitro
• Beneficiário: Maysa Santos Barbosa
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 16/23306-6 - Caracterização funcional e avaliação da antigenicidade e imunogenicidade de proteínas recombinantes de Mycoplasma agalactiae
• Beneficiário: Maysa Santos Barbosa
• Modalidade de apoio: Bolsas no Brasil - Doutorado