Multiple LEDT wavelengths modulate the Akt signaling pathways and attenuate pathological events in mdx dystrophic muscle cells

Autor(es)

da Rocha, Guilherme Luiz ; Mizobuti, Daniela Sayuri ; Mariano da Silva, Heloina Nathallie ; Covatti, Caroline ; de Lourenco, Caroline Caramano ; Salvador, Marcos Jose ; Leite Pereira, Elaine Cristina ; Minatel, Elaine

• Tipo de documento: Artigo Científico
• Fonte: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. N/A, p. 16-pg., 2022-04-05.

Resumo

This study is aimed at investigating the effects of LEDT, at multiple wavelengths, on intracellular calcium concentration; on transient receptor potential canonical channels; on calcium-binding protein; on myogenic factors; on myosin heavy chains; on Akt signaling pathway; on inflammatory markers; and on the angiogenic-inducing factor in dystrophic muscle cell culture experimental model. Dystrophic primary muscle cells were submitted to LEDT, at multiple wavelengths (420 nm, 470 nm, 660 nm, and 850 nm), and evaluated after 48 h for cytotoxic effects and intracellular calcium content. TRPC-1, TRPC-6, Calsequestrin, MyoD, Myogenin, MHC-slow, MHC-fast, p-AKT, p-mTOR, p-FoxO1, Myostatin, NF-kappa B, TNF-alpha, and VEGF levels were evaluated in dystrophic primary muscle cells by western blotting. The LEDT, at multiple wavelengths, treatedmdx muscle cells showed no cytotoxic effect and significant lower levels in [Ca2 +]i. The mdx muscle cells treated with LEDT showed a significant reduction of TRPC-1, NF-kappa B, TNF-alpha and MyoD levels and a significant increase of Myogenin, MHC-slow, p-AKT, p-mTOR, p-FoxO1 levels, and VEGF levels. Our findings suggest that different LEDT wavelengths modulate the Akt-signaling pathways and attenuate pathological events in dystrophic muscle cells, and a combined multiwavelength irradiation protocol may even provide a potentially therapeutic strategy for muscular dystrophies. [GRAPHICS] . (AU)

• Processo FAPESP: 20/09733-4 - Efeito das terapias de fotobiomodulação e antioxidante sobre eventos celulares, teciduais, moleculares e bioquímicos nas fibras musculares distróficas de camundongos Mdx
• Beneficiário: Elaine Minatel
• Modalidade de apoio: Auxílio à Pesquisa - Regular