Interaction of triblock copolymers (Pluronic (R)) with DMPC vesicles: a photophysical and computational study

Autor(es)

Calori, Italo Rodrigo ; Pinheiro, Lukas ; Braga, Gustavo ; Pedroso de Morais, Flavia Amanda ; Caetano, Wilker ; Tedesco, Antonio Claudio ; Hioka, Noboru

• Tipo de documento: Artigo Científico
• Fonte: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 275, p. 10-pg., 2022-07-05.

Resumo

Pluronic/lipid mix promises stealth liposomes with long circulation time and long-term stability for pharmaceutical applications. However, the influence of Pluronics on several aspects of lipid membranes has not been fully elucidated. Herein it was described the effect of Pluronics on the structured water, alkyl chain conformation, and kinetic stability of dimyristoylphosphatidylcholine (DMPC) liposomes using interfacial and deeper fluorescent probes along with computational molecular modeling data. Interfacial water changed as a function of Pluronics' hydrophobicity with polypropylene oxide (PPO) anchoring the copolymers in the lipid bilayer. Pluronics with more than 30-40 PO units had facilitated penetration at the bilayer while shorter PPO favored a more interfacial interaction. Low Pluronic concentrations provided long-term stability of vesicles by steric effects of polyethylene oxide (PEO), but high amounts destabilized the vesicles as a sum of water-bridge cleavage at the polar head group and the reduced alkyl-alkyl interactions among the lipids. The high kinetic stability of Pluronic/DMPC vesicles is a proof-of-concept of its advantages and applicability in nanotechnology over conventional liposome-based pharmaceutical products for future biomedical applications. (C) 2022 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 20/04507-6 - "hidrogéis confinados em matriz impressa 3d para a entrega controlada e sítio-específica de ingenol-3-dodecanoato e fluorocromo visando fotodiagnóstico e terapia de tumores cerebrais"
• Beneficiário: Italo Rodrigo Calori
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático