Peptide inhibitors of angiotensin-I converting enzyme based on angiotensin (1-7) with selectivity for the C-terminal domain

Autor(es)

Silva, Rogerio L. da ; Papakyriakou, Athanasios ; Carmona, Adriana K. ; Spyroulias, Georgios A. ; Sturrock, Edward D. ; Bersanetti, Patricia A. ; Nakaie, Clovis R.

• Tipo de documento: Artigo Científico
• Fonte: BIOORGANIC CHEMISTRY; v. 129, p. 11-pg., 2022-10-25.

Resumo

The renin-angiotensin system (RAS) is a key regulator of human arterial pressure. Several of its effects are modulated by angiotensin II, an octapeptide originating from the action of angiotensin-I converting enzyme (ACE) on the decapeptide angiotensin-I. ACE possess two active sites (nACE and cACE) that have their own kinetic and substrate specificities. ACE inhibitors are widely used as the first-line treatment for hypertension and other heart-related diseases, but because they inactivate both ACE domains, their use is associated with serious side effects. Thus, the search for domain-specific ACE inhibitors has been the focus of intense research. Angiotensin (1-7), a peptide that also belongs to the RAS, acts as a substrate of nACE and an inhibitor of cACE. We have synthetized 15 derivatives of Ang (1-7), sequentially removing the N-terminal amino acids and modifying peptides extremities, to find molecules with improved selectivity and inhibition properties. Ac-Ang (2-7)-NH2 is a good ACE inhibitor, resistant to cleavage and with improved cACE selectivity. Molecular dynamics simulations provided a model for this peptide's selectivity, due to Val3 and Tyr4 interactions with ACE subsites. Val3 has an important interaction with the S3 subsite, since its removal greatly reduced peptide-enzyme interactions. Taken together, our findings support ongoing studies using insights from the binding of Ac-Ang (2-7)-NH2 to develop effective cACE inhibitors. (AU)

• Processo FAPESP: 18/23269-9 - Estudos sintéticos e estruturais de peptídeos e polímeros: do aprimoramento da metodologia de síntese e da procura de derivados peptídicos para aplicações biotecnológicas
• Beneficiário: Clovis Ryuichi Nakaie
• Modalidade de apoio: Auxílio à Pesquisa - Regular