Co-immobilization of multiple enzymes on ferromagnetic nanoparticles for the depolymerization of xyloglucan

Autor(es)

Soares, Jessica M. ; Carneiro, Lara A. B. C. ; Barreto, Matheus Q. ; Ward, Richard J.

• Tipo de documento: Artigo Científico
• Fonte: BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR; v. 16, n. 6, p. 14-pg., 2022-08-02.

Resumo

Xyloglucan (XG) is an abundant polysaccharide in plant cell walls and some seeds and requires at least four different enzymes for complete depolymerization. Recombinant xyloglucanase from Aspergillus niveus (XegA), alpha-xylosidase from Escherichia coli (Yicl), beta-galactosidase from Hypocrea jecorina (Bga1) and beta-glucosidase from H. jecorina (Bgl1) were covalently immobilized individually and in combination on chitosan-coated ferromagnetic iron oxide nanoparticles functionalized with glutaraldehyde. All immobilized enzymes presented reduced specific catalytic activity, where immobilized Yicl, XegA, Bga1 and Bgl1 retained 14.9, 76.9, 29.5 and 6.6% activity as compared with the free enzymes, respectively. Immobilized and free enzymes presented similar optimum catalytic pH and optimum catalytic temperatures differed by +/- 10 degrees C between the free and immobilized enzymes. Bga1 and Bgl1 presented decreased maximum catalytic temperatures (T-opt), while Yicl presented an increased T-opt. The T(opt )of XegA remained unaltered. Mass spectrometry confirmed that nanoparticles carrying all four co-immobilized enzymes degraded XG to glucose, galactose and xylose, and higher proportions of co-immobilized Bg11 and XegA resulted in higher XG saccharification. Although levels of Bg11 activity were limiting, five re-use cycles of the co-immobilized enzymes were demonstrated, providing proof-of-principle for the use of a four-component multienzyme nanoparticle in the breakdown of a complex polysaccharide. (C) 2022 Society of Chemical Industry and John Wiley & Sons, Ltd. (AU)

• Processo FAPESP: 16/24139-6 - Desenho e Caracterização de Enzimas Xilanolíticas Multifuncionais
• Beneficiário: Richard John Ward
• Modalidade de apoio: Auxílio à Pesquisa - Regular
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• Beneficiário: Jéssica de Moura Soares
• Modalidade de apoio: Bolsas no Brasil - Mestrado
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• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Temático
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• Beneficiário: Lara Aparecida Buffoni de Campos Carneiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado
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• Beneficiário: Lara Aparecida Buffoni de Campos Carneiro
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado