| Texto completo | |
| Autor(es): |
Soares, Jessica M.
;
Carneiro, Lara A. B. C.
;
Barreto, Matheus Q.
;
Ward, Richard J.
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR; v. 16, n. 6, p. 14-pg., 2022-08-02. |
| Resumo | |
Xyloglucan (XG) is an abundant polysaccharide in plant cell walls and some seeds and requires at least four different enzymes for complete depolymerization. Recombinant xyloglucanase from Aspergillus niveus (XegA), alpha-xylosidase from Escherichia coli (Yicl), beta-galactosidase from Hypocrea jecorina (Bga1) and beta-glucosidase from H. jecorina (Bgl1) were covalently immobilized individually and in combination on chitosan-coated ferromagnetic iron oxide nanoparticles functionalized with glutaraldehyde. All immobilized enzymes presented reduced specific catalytic activity, where immobilized Yicl, XegA, Bga1 and Bgl1 retained 14.9, 76.9, 29.5 and 6.6% activity as compared with the free enzymes, respectively. Immobilized and free enzymes presented similar optimum catalytic pH and optimum catalytic temperatures differed by +/- 10 degrees C between the free and immobilized enzymes. Bga1 and Bgl1 presented decreased maximum catalytic temperatures (T-opt), while Yicl presented an increased T-opt. The T(opt )of XegA remained unaltered. Mass spectrometry confirmed that nanoparticles carrying all four co-immobilized enzymes degraded XG to glucose, galactose and xylose, and higher proportions of co-immobilized Bg11 and XegA resulted in higher XG saccharification. Although levels of Bg11 activity were limiting, five re-use cycles of the co-immobilized enzymes were demonstrated, providing proof-of-principle for the use of a four-component multienzyme nanoparticle in the breakdown of a complex polysaccharide. (C) 2022 Society of Chemical Industry and John Wiley & Sons, Ltd. (AU) | |
| Processo FAPESP: | 16/24139-6 - Desenho e Caracterização de Enzimas Xilanolíticas Multifuncionais |
| Beneficiário: | Richard John Ward |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/14452-1 - Um sistema biomimético nanoestruturado para a hidrólise enzimática de xiloglucano |
| Beneficiário: | Jéssica de Moura Soares |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol |
| Beneficiário: | Marcos Silveira Buckeridge |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 12/24147-8 - Desenho racional de enzimas multifuncionais para despolimerização do material lignocelulósico |
| Beneficiário: | Lara Aparecida Buffoni de Campos Carneiro |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 16/15708-7 - Caracterização de um novo produto de hidrólise do xiloglucano resultante da ação sinérgica entre uma mistura de beta-galactosidase/alfa-xilosidase |
| Beneficiário: | Lara Aparecida Buffoni de Campos Carneiro |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |