Potential of Pseudomonas and Trichoderma from the Brazilian Amazon as Biocontrol Agents against the Wheat Blast Disease

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de Azambuja Pereira, Maikon Richer ; Moreira, Silvino Intra ; da Silva, Abimael Gomes ; Nunes, Tiago Calves ; Campos Vicentini, Samara Nunes ; da Silva, Davi Prata ; da Silveira, Patricia Ricardino ; Kiyama de Oliveira, Tamiris Yoshie ; Silva, Tatiane Carla ; dos Santos Botelho, Deila Magna ; Vilela Resende, Mario Lucio ; Ceresini, Paulo Cezar

• Tipo de documento: Artigo Científico
• Fonte: AGRONOMY-BASEL; v. 12, n. 9, p. 17-pg., 2022-09-01.

Resumo

Blast is one of the most significant wheat diseases, causing high yield losses in susceptible varieties under favorable conditions in Latin America, Southeastern Asia and Eastern Africa. The disease is caused by the ascomycetous fungal pathogen Pyricularia oryzae Triticum lineage (PoTl). Chemical control with fungicides has been used as a management strategy; however, the effectiveness of the major classes of high-risk site-specific systemic fungicides has been reduced due to the widespread prevalence of resistance, especially in Brazil. Biological control is seen as a highly important and sustainable strategy to minimize the impact of yield losses associated with wheat blast in areas where fungicides are ineffective. In our study, we specifically aimed to determine the biological control potential of the three isolates of fluorescent Pseudomonas and three of Trichoderma as the antagonists of PoTl, both in in vitro and under greenhouse conditions. Additionally, we aimed to describe the ultrastructural interactions among the biocontrol agents and the pathogen in vitro by means of scanning electron microscopy (SEM). Fluorescent P. wayambapalatensis 'Amana' or Pseudomonas sp. nov. 'Yara', both from the P. putida group, and Trichoderma koningiopsis 'Cachara' significantly reduced PoTl in vitro mycelial growth and the blast disease severity on wheat plants. The SEM analyses revealed ultrastructural antagonistic mechanisms: biofilm formation, direct antagonism and mycoparasitism. Further research on the topic should include the development of stable formulations of the Pseudomonas- and Trichoderma-based biocontrol agents selected in our study for managing the wheat blast disease and the field tests of the biofungicide formulations obtained thereafter. (AU)

• Processo FAPESP: 18/21197-0 - Novas ferramentas de monitoramento de doenças e de resistência a fungicidas, em tempo real, para promover plataforma inteligente e sustentável de proteção de culturas no Brasil
• Beneficiário: Paulo Cezar Ceresini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/12509-1 - Novas ferramentas de monitoramento de doenças e de resistência a fungicidas, em tempo real, para promover plataforma inteligente e sustentável de proteção de culturas no Brasil
• Beneficiário: Silvino Intra Moreira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 20/07611-9 - Desenvolvimento de uma plataforma conjunta para monitoramento de epidemias e detecção de resistência a fungicidas visando a criação de um programa inteligente de manejo integrado da brusone do trigo no Paraná e em São Paulo
• Beneficiário: Paulo Cezar Ceresini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/01675-5 - Potencial de Pseudomonas e Trichoderma como agentes de biocontrole da brusone do trigo
• Beneficiário: Maikon Richer de Azambuja Pereira
• Modalidade de apoio: Bolsas no Brasil - Mestrado