Leaf tissue metabolomics fingerprinting of Citronella gongonha Mart. by H-1 HR-MAS NMR

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Ali, Sher ; Badshah, Gul ; Ali, Umar ; Afridi, Muhammad Siddique ; Shamim, Anwar ; Khan, Ajmir ; Felipe Soares, Frederico Luiz ; Alencar Menezes, Leociley Rocha ; Rezende, Vanessa Theodoro ; Barison, Andersson ; Fernandes de Oliveira, Carlos Augusto ; Tonin, Fernando Gustavo

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 12, n. 1, p. 13-pg., 2022-10-21.

Resumo

This research characterizes key metabolites in the leaf from Citronella gongonha Martius (Mart.) Howard (Cardiopteridaceae). All metabolites were assessed in intact leaf tissue by proton (H-1) high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy integrated with the principal component analysis (PCA) to depict molecular association with the seasonal change. The major 'known unknown' metabolites detected in H-1 HR-MAS NMR were derivatives of flavonoid, polyphenolic and monoterpenoid compounds such as kaempferol-3-O-dihexoside, caffeoyl glucoside (2), 3-O-caffeoylquinic acid (3), 5-O-caffeoylquinic acid (4), kingiside (5), 8-epi-kingisidic acid (6), (7 alpha)-7-O-methylmorroniside (7), (7 beta)-7-O-methylmorroniside (8) and alpigenoside (9) together with the universally occurring sucrose (10), alpha-glucoses (11, 12), alanine (13), and fatty (linolenic) acid (14). Several of the major metabolites (1, 2-9) were additionally confirmed by liquid chromatography tandem mass spectrometry (LC-MS/MS). In regard with the PCA results, metabolites 1, 2-9 and 14 were influenced by seasonal variation and/or from further (a) biotic environmental conditions. The findings in this work indicate that C. gongonha Mart. is an effective medicinal plant by preserving particularly compounds 2, 3-9 in abundant amounts. Because of close susceptibility with seasonal shift and ecological trends, further longitudinal studies are needed to realize the physiology and mechanism involved in the production of these and new metabolites in this plant under controlled conditions. Also, future studies are recommended to classify different epimers, especially of the phenolics and monoterpenoids in the given plant. (AU)

• Processo FAPESP: 22/03952-1 - Avaliação da exposição de crianças às micotoxinas através da dieta em Ribeirão Preto/SP
• Beneficiário: Sher Ali
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado