| Texto completo | |
| Autor(es): |
Dias, Luana Mendonca
;
Klein, Marlise Inez
;
Ferrisse, Tulio Morandin
;
Medeiros, Karine Sousa
;
Jordao, Claudia Carolina
;
Bellini, Amanda
;
Pavarina, Ana Claudia
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF FUNGI; v. 9, n. 1, p. 22-pg., 2023-01-01. |
| Resumo | |
This study aimed to evaluate the potential of successive applications of sub-lethal doses of the antimicrobial photodynamic therapy (aPDT) mediated by Photodithazine (R) (PDZ) and curcumin (CUR) associated with LED in the viability, reactive oxygen species (ROS) production, and gene expression of Candida albicans. The microbial assays were performed using planktonic cultures and biofilms. Ten successive applications (Apl#) were performed: aPDT (P+L+; C+L+), photosensitizer (P+L-; C+L-), and LED (P-L+; C-L+). Control groups were used (P-L-; C-L-). The viability of C. albicans was determined by cultivating treated cultures on agar plates with or without fluconazole (FLU). In addition, the ROS detection and expression of SOD1, CAP1, and ERG11 genes were determined. For planktonic cultures, no viable colonies were observed after Apl#3 (without FLU) and Apl#2 (with FLU) for either photosensitizer. Biofilm treated with P+L+ resulted in the absence of cell viability after Apl#7, while C+L+ showed similar to 1.40 log(10) increase in cell viability after Apl#2, regardless of FLU. For both photosensitizers, after the last application with viable colonies, the production of ROS was higher in the biofilms than in the planktonic cultures, and SOD1 expression was the highest in P+L+. A reduction of CAP1 and ERG11 expression occurred after P+L+, regardless of FLU. C+L+ had a higher level of ROS, and the treatments were non-significant for gene expression. Sub-lethal doses of aPDT mediated by CUR could induce C. albicans resistance in biofilms, while C. albicans cells in biofilms were susceptible to aPDT mediated by PDZ. (AU) | |
| Processo FAPESP: | 18/14874-6 - Potencial da terapia fotodinâmica antimicrobiana no desenvolvimento de resistência em Candida albicans |
| Beneficiário: | Luana Mendonça Dias Santana |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 19/27634-6 - Avaliação do efeito da terapia fotodinâmica antimicrobiana associada a DNAse no tratamento de Candidose Oral em camundongos infectados com Candida albicans |
| Beneficiário: | Cláudia Carolina Jordão |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 21/01191-0 - Indução de tolerância e caracterização do biofime de Candida albicans submetido à terapia fotodinâmica antimicrobiana |
| Beneficiário: | Túlio Morandin Ferrisse |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica |
| Beneficiário: | Vanderlei Salvador Bagnato |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |