| Texto completo | |
| Autor(es): |
Zuardi, Leonardo Raphael
;
de Oliveira, Fabiola Singaretti
;
Fernandes, Roger Rodrigo
;
Oliveira Gomes, Maria Paula
;
Spriano, Silvia
;
Nanci, Antonio
;
de Oliveira, Paulo Tambasco
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOMIMETICS; v. 7, n. 3, p. 15-pg., 2022-09-01. |
| Resumo | |
This study evaluates the effects of the availability of exogenous BMP-7 on osteoblastic cells' differentiation on a nanotextured Ti surface obtained by chemical etching (Nano-Ti). The MC3T3-E1 and UMR-106 osteoblastic cell lines were cultured for 5 and 7 days, respectively, on a Nano-Ti surface and on a control surface (Control-Ti) in an osteogenic medium supplemented with either 40 or 200 ng/mL recombinant mouse (rm) BMP-7. The results showed that MC3T3-E1 cells exhibited distinct responsiveness when exposed to each of the two rmBMP-7 concentrations, irrespective of the surface. Even with 40 ng/mL rmBMP-7, important osteogenic effects were noticed for Control-Ti in terms of cell proliferation potential; Runx2, Osx, Alp, Bsp, Opn, and Smad1 mRNA expression; and in situ ALP activity. For Nano-Ti, the effects were limited to higher Alp, Bsp, and Opn mRNA expression and in situ ALP activity. On both surfaces, the osteogenic potential of UMR-106 cultures remained unaltered with 40 ng/mL rmBMP-7, but it was significantly reduced when the cultures were exposed to the 200 ng/mL concentration. The availability of rmBMP-7 to pre-osteoblastic cells at the concentrations used alters the expression profile of osteoblast markers, indicative of the acquisition of a more advanced stage of osteoblastic differentiation. This occurs less pronouncedly on the nanotextured Ti and without reflecting in higher mineralized matrix production by differentiated osteoblasts on both surfaces. (AU) | |
| Processo FAPESP: | 16/50298-4 - Funcionalização de superfície de materiais bioativos com moléculas naturais para a modulação da resposta tecidual a implantes aloplásticos |
| Beneficiário: | Paulo Tambasco de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |