| Texto completo | |
| Autor(es): Mostrar menos - |
Claro, Amanda Maria
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Do Amaral, Nayara Cavichiolli
;
Medalha Colturato, Vitoria Maria
;
Aleixo, Nadia Andrade
;
Paiva, Robert
;
Cruz, Sandra Andrea
;
Monteiro, Gustavo Claro
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Goncalves De Carvalho, Gustavo Senra
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Resende Nogueira, Flavia Aparecida
;
Deffune, Elenice
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da Costa Iemma, Monica Rosas
;
Barud, Hernane da Silva
Número total de Autores: 12
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| Tipo de documento: | Artigo Científico |
| Fonte: | Cellulose; v. 29, n. 18, p. 12-pg., 2022-10-16. |
| Resumo | |
Cellulose is a versatile tunable material that finds application in the biomedical field as substrate for cell culture. However, few studies have been done to explore the use of bacterial cellulose (BC), a naturally occurring nanofibrillar material with distinct properties, as a platform to produce such device. In the present work, BC membranes have been functionalized with thiol functional group (SH) through silanization reaction with (3-mercaptopropyl)trimethoxysilane (MPTMS) under different conditions, in order to obtain a platform with improved cell adhesion. The efficiency of BC surface modification with MPTMS was evaluated by using acid and base catalyzed reactions, and two different drying methods: at room temperature of 28 degrees C and at 120 degrees C as curing temperature. The results of the set of analyses performed-ATR-FTIR, TGA, elemental analysis,C-13 NMR, contact angle and SEM-indicate that BC surface functionalization was efficient, regardless the drying process. The MPTMS-modified platforms exhibited sulfur content of 3-5 times higher than native BC. The performed biological assay with fibroblast GM07492 human cells revealed that adhesion of cells to the BC surface depends not only on the functional group present at the matrix but also on surface wettability. (AU) | |
| Processo FAPESP: | 20/05163-9 - Celulose bacteriana/gelatina como plataforma biopolimérica para aplicação em cultivo celular |
| Beneficiário: | Nayara Cavichiolli do Amaral |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 19/12711-5 - Investigação do efeito da modificação da celulose bacteriana com organossilanos na adesão de fibroblastos humanos |
| Beneficiário: | Nayara Cavichiolli do Amaral |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |