The influence of parental high-fat high-sugar diet on the gut-brain axis in male offspring

Autor(es)

Cesar, Helena ; Sertorio, Marcela Nascimento ; Santamarina, Aline ; de Souza, Esther Alves ; Mennitti, Lais Valles ; Jamar, Giovana ; Juca, Andrea ; Casagrande, Breno Picin ; Estadela, Debora ; Pisani, Luciana Pellegrini

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 160, p. 11-pg., 2022-07-26.

Resumo

Purpose: The gut-brain axis (GBA) is implicated in the development of obesity, and its role in developmental programming needs to be explored. This study uncovers the effects of a parental high-fat, high-sugar diet (HFS) on the gut (colon) and brain (hypothalamus) GBA of male Wistar rat offspring at weaning until adulthood. Methods: For ten weeks before mating, male progenitors were fed a control diet (CD) or HFS, whereas dams were fed CD or HFS during pregnancy and lactation. Male offspring aged 21-and 90-day old were assessed for: Gene expression of toll-like receptor 4 (TLR4) pathway and zonula occludens 1 (ZO1) in the colon and hypothalamus; hypothalamic gene expression of orexigenic neuropeptides and Leptin receptor; serum levels of lipopolysac-charide (LPS), glucagon like peptide 1 (GLP-1), Ghrelin and neuropeptide Y (NPY); colonic cytokine levels; Faecal Bifidobacterium spp. and Lactobacillus spp. DNA. Results: Paternal HFS showed increased endotoxaemia, reduced colonic gene expression of ZO1 and reduced colonic TNF-alpha at weaning. In the adult offspring, paternal HFS showed increased NPY, reduced serum Ghrelin, colonic pro-inflammatory cytokines, and lower faecal Bifidobacterium spp. DNA. Maternal diet showed increased hypothalamic gene expression of myeloid differentiation primary response 88 (MYD88) at weaning. The maternal HFS diet showed increased NPY and reduced faecal Bifidobacterium spp. and Lactobacillus spp. DNA in the adult offspring. The combined effect of parental diet showed increased NPY at weaning, and low-er Bifidobacterium spp. and Lactobacillus spp. in the adult offspring. Conclusion: Maternal and paternal HFS diet seem to influence the programming of the gut-brain axis, leading to increased visceral adiposity and weight of male offspring at weaning, the effect that lasted until adulthood. (AU)

• Processo FAPESP: 17/09646-1 - Efeitos da dieta materna e paterna na programação metabólica da prole com 21 dias até a idade adulta
• Beneficiário: Helena de Cássia César Vallera
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 19/09724-8 - Influência da ingestão materna e paterna de dieta hiperlipídica/hiperglicídica no eixo cólon-hipotálamo da prole no início da vida adulta
• Beneficiário: Luciana Pellegrini Pisani
• Modalidade de apoio: Auxílio à Pesquisa - Regular