| Texto completo | |
| Autor(es): |
Alexander Bruck, Stefan
;
Contato, Alex Graca
;
Gamboa-Trujillo, Paul
;
de Oliveira, Tassio Brito
;
Cereia, Mariana
;
de Moraes Polizeli, Maria de Lourdes Teixeira
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MICROORGANISMS; v. 10, n. 2, p. 15-pg., 2022-02-01. |
| Resumo | |
The isolation of filamentous fungal strains from remote habitats with extreme climatic conditions has led to the discovery of a series of enzymes with attractive properties that can be useful in various industrial applications. Among these, cold-adapted enzymes from fungi with psychrotrophic lifestyles are valuable agents in industrial processes aiming towards energy reduction. Out of eight strains isolated from soil of the paramo highlands of Ecuador, three were selected for further experimentation and identified as Cladosporium michoacanense, Cladosporium sp. (cladosporioides complex), and Didymella sp., this last being reported for the first time in this area. The secretion of seven enzymes, namely, endoglucanase, exoglucanase, beta -D-glucosidase, endo-1,4-beta -xylanase, beta -D-xylosidase, acid, and alkaline phosphatases, were analyzed under agitation and static conditions optimized for the growth period and incubation temperature. Cladosporium strains under agitation as well as incubation for 72 h mostly showed the substantial activation for endoglucanase reaching up to 4563 mU/mL and xylanase up to 3036 mU/mL. Meanwhile, other enzymatic levels varied enormously depending on growth and temperature. Didymella sp. showed the most robust activation at 8 degrees C for endoglucanase, beta -D-glucosidase, and xylanase, indicating an interesting profile for applications such as bioremediation and wastewater treatment processes under cold climatic conditions. (AU) | |
| Processo FAPESP: | 18/07522-6 - Desenvolvimento de tecnologias ecológicas para a hidrólise de biomassa e resíduos da indústria de celulose e papel |
| Beneficiário: | Maria de Lourdes Teixeira de Moraes Polizeli |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 08/57908-6 - Instituto Nacional de Biotecnologia para o Bioetanol |
| Beneficiário: | Marcos Silveira Buckeridge |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |
| Processo FAPESP: | 17/25862-6 - Estudos estruturais e propriedades funcionais de xiloglucanases e liquenases fúngicas: aplicações na hidrólise de biomassa lignocelulósica |
| Beneficiário: | Alex Graça Contato |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 17/09000-4 - Efeitos da temperatura e da deficiência hídrica, em cenário climático futuro, na comunidade de fungos em solo tropical e na seleção de linhagens produtoras de óxido nitroso |
| Beneficiário: | Tássio Brito de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol |
| Beneficiário: | Marcos Silveira Buckeridge |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |