Latex-collagen membrane: an alternative treatment for tibial bone defects

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de Paiva, Marcela Britto ; Brasil, Giovana Sant'Ana Pegorin ; Chagas, Ana Laura Destro ; Macedo, Ana Paula ; Ramos, Junia ; Issa, Joao Paulo Mardegan ; Gangrade, Ankit ; Floriano, Juliana Ferreira ; Caetano, Guilherme Ferreira ; Li, Bingbing ; Farhadi, Neda ; Mandal, Kalpana ; Dokmeci, Mehmet Remzi ; Jucaud, Vadim ; Herculano, Rondinelli Donizetti ; Shimano, Antonio Carlos

• Tipo de documento: Artigo Científico
• Fonte: Journal of Materials Science; v. N/A, p. 23-pg., 2022-12-08.

Resumo

Due to the difficulty of consolidation, the risk of fractures, and the high cost of therapy, the orthopedic field views the restoration of bony defects or post-traumatic diseases as a significant issue. In recent years, efforts have been made toward guided bone regeneration as a viable solution. In this study, natural rubber latex (NRL) and latex-collagen (NRL-COL) membranes were developed and characterized, and their effects on bone regeneration in rat tibial lesions were assessed. According to the physicochemical findings, the hydrolyzed COL was effectively incorporated into the NRL membrane to produce a more robust membrane (348% increase in stress) and showed good biocompatibility for human dermal fibroblast cells and in hemolytic activity assay. According to bone analysis using computed microtomography, a substantial difference in bone volume (BV) was found among NRL, control (CONT), and NRL-COL. NRL-COL exhibited the greatest value for the bone volume/total volume ratio (BV/TV). The presence of NRL and COL was significant for the analysis of BV and BV/TV, respectively. All the experimental groups demonstrated a higher bone quality and fracture resistance, as shown by the mechanical properties of the bone. In the COL group, both neoformation of bone and total collagen levels were greater, indicating that treated defects had been closed and mature bone cells were present. Due to its angiogenic properties and the low cost of NRL membrane production, the NRL-COL membrane could be a good substitute for upcoming bone regeneration applications. (AU)

• Processo FAPESP: 14/17526-8 - Desenvolvimento de um novo sistema empregando o látex natural como liberador de extratos vegetais reforçados por nanopartículas: caracterização física, química e biológica
• Beneficiário: Rondinelli Donizetti Herculano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/17411-8 - Avaliação da liberação controlada de fármacos utilizando membranas de látex natural como carreador
• Beneficiário: Rondinelli Donizetti Herculano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/19603-8 - Desenvolvimento de um novo adesivo à base de látex natural reforçado com barbatimão e suas frações e estudo das vias de sinalização do reparo dérmico
• Beneficiário: Rondinelli Donizetti Herculano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/20051-0 - Regeneração de defeitos ósseos utilizando diferentes recursos terapêuticos
• Beneficiário: Antônio Carlos Shimano
• Modalidade de apoio: Auxílio à Pesquisa - Regular