Microbiome structure in biofilms from a volcanic island in Maritime Antarctica investigated by genome-centric metagenomics and metatranscriptomics

Autor(es)

Centurion, V. B. ; Campanaro, S. ; Basile, A. ; Treu, L. ; Oliveira, V. M.

• Tipo de documento: Artigo Científico
• Fonte: MICROBIOLOGICAL RESEARCH; v. 265, p. 11-pg., 2022-12-01.

Resumo

Antarctica is the coldest and driest continent on Earth, characterized by polyextreme environmental conditions, where species adapted form complex networks of interactions. Microbial communities growing in these harsh environments can form biofilms that help the associated species to survive and thrive. A rich body of knowledge describes environmental biofilm communities; however, most studies have focused on dominant community members rather than functional complexity and metabolic potential. To overcome these limitations, the present study used genome-centric metagenomics to describe two biofilm samples subjected to different temperature collected in Deception Island, Maritime Antarctica. The results unraveled a complex biofilm microbiome rep-resented by 180 metagenome-assembled genomes. The potential metabolic interactions were investigated using metabolic flux balance analysis and revealed that purple bacteria are the community members with the highest correlations with other bacteria. Due to their predicted mixotrophic behavior, they may play a crucial role in the microbiome, likely supporting the heterotrophic species in biofilms. Metatranscriptomics results revealed that the chaperone system and proteins counteracting ROS and toxic compounds have a major role in maintaining bacterial cell homeostasis in sediments of volcanic origin. (AU)

• Processo FAPESP: 19/22891-0 - Recuperação de genomas a partir de dados metagenômicos e análise funcional do microbioma do solo de Whalers Bay: amostras antárticas
• Beneficiário: Victor Borin Centurion Biruel
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 16/05640-6 - Multi-ômicas aplicadas ao conhecimento e exploração de microbiomas Antárticos
• Beneficiário: Valeria Maia Merzel
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/03172-8 - Mecanismos de adaptação ao estresse em microbioma de sedimentos da Ilha Vulcânica Deception na Antártica marítima
• Beneficiário: Victor Borin Centurion Biruel
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto