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Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection

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Autor(es):
Cavichioli, Thais Magni ; Curtolo, Maiara ; Cristofani-Yaly, Mariangela ; Rodrigues, Josiane ; Della Coletta-Filho, Helvecio
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: AGRONOMY-BASEL; v. 12, n. 10, p. 11-pg., 2022-10-01.
Resumo

Background: Huanglongbing (HLB) is currently one of the most devasting diseases in citrus plants worldwide. Resistance against its causal agent, Candidatus Liberibacter asiaticus (CLas), in commercial Citrus species remains a challenge, even though they show differences in CLas multiplication. Methods: A total of 14 citrandarins and their parents (Sunki mandarin and Poncirus trifoliata cv. Rubidoux) were top-grafted onto the canopy of potted 'Valencia' sweet orange plants with high CLas titers. The grafted genotypes were tested for CLas infection and physiological effects of the disease (starch accumulation and callose deposition) for 12 months. Results: All tested genotypes were infected by CLas during the time frame of the experiment. However, a decrease in the infection rate ranging from 50% to 80% for the hybrids H109, H126, H157, and H222 was observed 360 days from the top-grafting. CLas was undetected by real-time PCR in H106 at the end of the experiment, which had low levels of starch and callose deposition. Conclusions: CLas infected all of the tested citrandarins, but a decrease in the infection rate over time was detected for some specific genotypes, which led to less starch accumulation and callose deposition. (AU)

Processo FAPESP: 20/07045-3 - Estratégias biotecnológicas e genômicas para qualidade, produtividade e manejo sustentável de citros, café e cana-de-açúcar no estado de São Paulo
Beneficiário:Mariângela Cristofani-Yaly
Modalidade de apoio: Auxílio à Pesquisa - Núcleos de Pesquisa Orientada a Problemas em São Paulo
Processo FAPESP: 21/03989-0 - Recombinação homologa mediada pelo sistema CRISPR/Cas e inserção de gene de resistência no desenvolvimento de variedades de laranjas doce resistentes ao Huanglongbing
Beneficiário:Maiara Curtolo
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 19/06412-5 - Respostas de citrandarins (Poncirus trifoliata x tangerina Sunki) à infecção por "Candidatus Liberibacter asiaticus"
Beneficiário:Thais Magni Cavichioli
Modalidade de apoio: Bolsas no Brasil - Mestrado