Biological activity of pectic polysaccharides investigated through biomembrane models formed at the air-water interface

Autor(es)

Escobar, Bruno de Andrade ; Valerio, Gabriel Lundgren Ferreira ; Caseli, Luciano

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 216, p. 6-pg., 2022-05-12.

Resumo

Pectin, a polysaccharide with potential bioactivity, was inserted in the aqueous subphase of monolayers of selected lipids DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) and DPPE (1,2-dipalmitoyl-sn-glycero-3phosphoethanolamine), representing mammalian and bacterial membranes, respectively. Pectin condensed monolayers but made the DPPC monolayer more fluid, while for DPPE, it made its monolayer more rigid, detected with dynamic interfacial rheology. Complementary data using surface potential, infrared spectroscopy, and Brewster angle microscopy also showed distinctive effects of pectin on DPPE and DPPC. We believe these data can be correlated with the action of this polysaccharide with biological lipidic surfaces with different polar heads, which may be relevant, generally speaking, to understanding the molecular mechanism of this bioactive compound for pharmaceutical purposes. (AU)

• Processo FAPESP: 19/03239-0 - Interfaces nanoestruturadas para a investigação de substâncias bioativas em modelos de membrana celular e para a construção de dispositivos optoeletrônicos enzimáticos
• Beneficiário: Luciano Caseli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático