Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors

Autor(es)

Crulhas, Bruno P. ; Basso, Caroline R. ; Castro, Gustavo R. ; Pedrosa, Valber A.

• Tipo de documento: Artigo Científico
• Fonte: Current Medicinal Chemistry; v. 29, n. 37, p. 8-pg., 2022-01-01.

Resumo

Background: Prostate cancer cells have very high PCA3 messenger RNA levels, which turns them into one of the new biomarkers for prostate cancer prognosis and diagnosis. Objective: Our goal here is to develop a new aptasensor to detect PCA3 release by the cancer cell. Methods: DNA hairpin containing PCA3 aptamer was thiolated, conjugated to methylene blue (MB) redox probe, and immobilized on gold electrode through self-assembly to detect label-free cancer cells. Results: Our data have evidenced stable and sensitive sensors presenting a wide linear detection range (0-150ng/mL). In addition, monitoring PCA3 released by different types of prostate cells can provide in-depth knowledge about prostate cancer dynamics; therefore, it is a powerful platform for earlier clinical diagnostic. The released PCA3 can vary depending on the type of adopted prostate cells. Conclusion: PCA3 release was monitored in a group of cells for 2 h; it showed significantly higher expression in both LNCaP and PC-3 cells. This strategy provides a unique and simple methodology to achieve more sensitive and specific PCA3 detection; thus, it emerged as a promising tool for early cost-effective diagnosis (AU)

• Processo FAPESP: 19/13411-5 - Desenvolvimento de dispositivo microfluídico para biopsia líquida de células tumorais circulantes
• Beneficiário: Valber de Albuquerque Pedrosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular