| Texto completo | |
| Autor(es): |
de Oliveira, Amanda Aparecida
;
Ramalho, Manuela de Oliveira
;
Moreau, Corrie Saux
;
Campos, Ana Eugenia de Carvalho
;
Harakava, Ricardo
;
Bueno, Odair Correa
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MICROBIOLOGICAL RESEARCH; v. 263, p. 17-pg., 2022-07-19. |
| Resumo | |
The olive crop has expanded in the southeastern region of South America, particularly in Brazil. Thus, the objectives of this study were to identify the diversity of endophytic microorganisms associated with olive leaves with culture-dependent and culture-independent methods, to explore which factors influence the composition and abundance of this microbial community, to identify the trophic mode of these fungi by FunGuild and, to verify type associations between bacterial and fungal communities. Leaf samples were collected from 93 plants in nine locations in the Brazilian states of Sao Paulo and Minas Gerais. Leaves were first superficially disinfected before fungal isolation and next-generation metabarcoding sequencing was completed targeting the 16S rRNA regions for bacteria and ITS1 for fungi. In total, 800 isolates were obtained, which were grouped into 191 morphotypes and molecularly identified, resulting in 38 genera, 32 of which were recorded for the first time in cultivated olive trees in Brazil. For the isolated fungi, the most abundant trophic level was pathotrophic and for the culture-independent method was unidentified followed by symbiotrophic. The metabarcoding results revealed that factors such as plant age, altitudinal gradient, and geographic location can influence the microbial community of commercial olive plants, while the specific cultivar did not. (AU) | |
| Processo FAPESP: | 17/50334-3 - Plano de Desenvolvimento Institucional em Pesquisa (PDIp): modernização e adequação de unidades multiusuárias estratégicas do Instituto Biológico |
| Beneficiário: | Ana Eugênia de Carvalho Campos |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Modernização de Institutos Estaduais de Pesquisa |