New insights into cadmium tolerance and accumulation in tomato: Dissecting root and shoot responses using cross-genotype grafting

Autor(es)

Marques, Deyvid Novaes ; Nogueira, Marina Lima ; Gaziola, Salete Aparecida ; Batagin-Piotto, Katherine Derlene ; Freitas, Natalia Chagas ; Alcantara, Berenice Kussumoto ; Paiva, Luciano Vilela ; Mason, Chase ; Piotto, Fernando Angelo ; Azevedo, Ricardo Antunes

• Tipo de documento: Artigo Científico
• Fonte: Environmental Research; v. 216, p. 14-pg., 2023-01-01.

Resumo

Cadmium (Cd) is one of the most threatening soil and water contaminants in agricultural settings. In previous studies, we observed that Cd affects the metabolism and physiology of tomato (Solanum lycopersicum) plants even after short-term exposure. The objective of this research was to use cross-genotype grafting to distinguish be-tween root-and shoot-mediated responses of tomato genotypes with contrasting Cd tolerance at the early stages of Cd exposure. This study provides the first report of organ-specific contributions in two tomato genotypes with contrasting Cd tolerance: Solanum lycopersicum cv. Calabash Rouge and Solanum lycopersicum cv. Pusa Ruby (which have been classified and further characterized as sensitive (S) and tolerant (T) to Cd, respectively). Scion S was grafted onto rootstock S (S/S) and rootstock T (S/T), and scion T was grafted onto rootstock T (T/T) and rootstock S (T/S). A 35 mu M cadmium chloride (CdCl2) treatment was used for stress induction in a hydroponic system. Both shoot and root contributions to Cd responses were observed, and they varied in a genotype-and/or organ-dependent manner for nutrient concentrations, oxidative stress parameters, antioxidant enzymes, and transporters gene expression. The findings overall provide evidence for the dominant role of the tolerant root-stock system in conferring reduced Cd uptake and accumulation. The lowest leaf Cd concentrations were observed in T/T (215.11 mu g g-1 DW) and S/T (235.61 mu g g-1 DW). Cadmium-induced decreases in leaf dry weight were observed only in T/S (-8.20%) and S/S (-13.89%), which also were the only graft combinations that showed decreases in chlorophyll content (-3.93% in T/S and-4.05% in S/S). Furthermore, the results show that reciprocal grafting is a fruitful approach for gaining insights into the organ-specific modulation of Cd tolerance and accumulation during the early stages of Cd exposure. (AU)

• Processo FAPESP: 20/12666-7 - Modulação tecido-específica do proteoma, da síntese de fitoquelatinas e da atividade de enzimas antioxidantes de tomateiro em resposta ao Cd, utilizando enxertias recíprocas
• Beneficiário: Deyvid Novaes Marques
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 22/11018-7 - Metabolômica para resposta ao estresse induzido por cádmio: foco em plantas de tomateiro enxertadas
• Beneficiário: Deyvid Novaes Marques
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 17/05544-0 - Estudo da cinética de absorção e do acúmulo de cádmio em cultivares de tomateiro
• Beneficiário: Deyvid Novaes Marques
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/20706-9 - Identificação de proteínas-chave à tolerância ao cádmio: análise proteômica de cultivares de tomateiro sensível e tolerante ao Cd, e plantas enxertadas reciprocamente
• Beneficiário: Deyvid Novaes Marques
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 21/06117-3 - RouPAGEm enzimática do tomateiro e os mecanismos de tolerância ao estresse induzido por cádmio
• Beneficiário: Ricardo Antunes de Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/05788-3 - Caracterização de mecanismos de tolerância ao Cd em raízes de tomateiros usando porta-enxertos tolerantes
• Beneficiário: Katherine Derlene Batagin Piotto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/14349-3 - Transporte e acúmulo de cádmio em diferentes genótipos de tomateiro: abordagens fisiológicas, proteômicas e moleculares.
• Beneficiário: Ricardo Antunes de Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular