Biotransformations of nitriles mediated by in vivo nitrile hydratase of Rhodococcus erythropolis ATCC 4277 heterologously expressed in E. coli

Autor(es)

Moraes, Maraylla I. ; Iglesias, Cesar ; Teixeira, Iris S. ; Milagre, Humberto M. S. ; Giordano, Sonia Rodriguez ; Milagre, Cintia D. F.

• Tipo de documento: Artigo Científico
• Fonte: RESULTS IN CHEMISTRY; v. 5, p. 7-pg., 2023-01-03.

Resumo

Nitrile hydratase activity has been reported as an exciting alternative for the industrial production of a variety of compounds overwhelming its chemical counterpart; despite this, until now, a few enzymes have been thoroughly studied. Efficient expression of nitrile hydratase enzymes has been the bottleneck to explore this activity. Here, we report the cloning and expression of Rhodococcus erythropolis ATCC 4277 nitrile hydratase (alpha-and beta-subunits) and the correspondent activator gene. Furthermore, substrate scope with whole cells of recombinant E. coli demonstrates that this Fe-type NHase could hydrate a wide range of aliphatic and aromatic nitrile with high conversion rates and moderate enantiomeric excess. (AU)

• Processo FAPESP: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock
• Beneficiário: Arlene Gonçalves Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia
• Processo FAPESP: 19/15230-8 - Resolução cinética dinâmica quimioenzimática de álcoois terciários
• Beneficiário: Humberto Márcio Santos Milagre
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50926-0 - INCT 2014: biodiversidade e produtos naturais
• Beneficiário: Vanderlan da Silva Bolzani
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático