Transcription Factor AhR, Cytokines IL-6 and IL-22 in Subjects with and without Peri-Implantitis: A Case Control-Study

Autor(es)

Martins, Luis Ricardo Linard ; Grzech-Lesniak, Kinga ; Castro dos Santos, Nidia ; Suarez, Lina J. ; Giro, Gabriela ; Bastos, Marta Ferreira ; Shibli, Jamil Awad

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH; v. 19, n. 12, p. 10-pg., 2022-06-01.

Resumo

Peri-implantitis is a plaque-associated condition characterized by mucosal inflammation and subsequent progressive loss of supporting bone; it is caused by bacterial biofilm, but the host response triggered by bacterial stimulation promotes the release of cells and mediators that culminate in tissue destruction. The Aryl-hydrocarbon Receptor (AhR) is associated with IL-22 production by Th22 and Th17 CD4+ Th cells. The presence of IL-6 may promote the Th22 phenotype. The present case-control study evaluated the gene expression of AhR, IL-22, and IL-6 in the peri-implant tissues of healthy and peri-implantitis patients. Tissue biopsies were collected from thirty-five volunteers (15 healthy and 20 with peri-implantitis). A real-time PCR reaction was utilized to assess the AhR, IL-22, and IL-6 gene expression levels relative to the reference gene (GAPDH). The results were analyzed using the Mann-Whitney test with a significance level of 5%. Higher levels of gene expression of AhR and IL-6 were detected in peri-implantitis tissues. The IL-22 gene expression levels did not differ between groups. In conclusion, higher gene expression levels for AhR and IL-6 were detected in the soft tissues of peri-implantitis patients. IL-22 did not vary between conditions, which may indicate the loss of the immunomodulatory role of IL-22 in periimplantitis. (AU)

• Processo FAPESP: 08/07154-5 - Avaliação imunológica do tratamento cirúrgico da periimplantite utilizando Er,Cr:YSGG associado a antibioticoterapia sistêmica.
• Beneficiário: Luciana Aparecida de Gouveia Cardoso
• Modalidade de apoio: Bolsas no Brasil - Mestrado