Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production

Autor(es)

Pereira Scarpa, Josiani de Cassia ; Marques, Natalia Paganini ; Monteiro, Diego Alves ; Martins, Gisele Marta ; de Paula, Ariela Veloso ; Boscolo, Mauricio ; da Silva, Roberto ; Gomes, Eleni ; Bocchini, Daniela Alonso

• Tipo de documento: Artigo Científico
• Fonte: INDUSTRIAL CROPS AND PRODUCTS; v. 141, p. 8-pg., 2019-12-01.

Resumo

Pycnoporus swsguineus MCA 16 was cultivated by solid state fermentation on lignocellulosic residues, at 40 degrees C. The highest endoglucanase, exoglucanase, beta-glucosidase, xylanase, beta-xylosidase and laccase activities (200, 29.1, 52.1, 44.2, 1.81 and 22.04 U g(-1), respectively) were obtained when using the mixture of wheat bran and soybean meal (1:1 w/w) as substrates, at 96 h of cultivation. Peroxidases highest activities were observed at 48 h (14.61 and 10.02 U g(-1) for manganese and lignin peroxidase, respectively). Enzymatic saccharification of sugarcane bagasse submitted to hydrothermal alkaline pretreatment provided glucose at 7.32 g L-1 when using solid load of 13.5% (dry basis), 288.3 total units of endoglucanase/g of cellulose, 130 h and 57 degrees C. Ligninases present in the enzymatic solution provided a reduction of 82.3% in the concentration of total phenolic compounds during saccharification. The hydrolysate was used for ethanol production by Saccharomyces cerevisiae CAT-1 and a yield of 73.5% was obtained. (AU)

• Processo FAPESP: 11/19554-0 - Prospecção de fungos filamentosos termofílicos produtores de celulases e aplicação das enzimas na sacarificação do bagaço de cana-de-açúcar
• Beneficiário: Daniela Alonso Bocchini
• Modalidade de apoio: Auxílio à Pesquisa - Regular