Production of sugars from mixed hardwoods for use in the synthesis of sugar fatty acid esters catalyzed by immobilized-stabilized derivatives of Candida antarctica lipase B

Autor(es)

Goncalves, Maria Carolina Pereira ; Cansian, Ana Barbara Moulin ; Tardioli, Paulo Waldir ; Saville, Bradley A.

• Tipo de documento: Artigo Científico
• Fonte: BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR; v. 17, n. 5, p. 15-pg., 2023-06-19.

Resumo

The synthesis of sugar fatty acid esters (SFAEs) from lignocellulosic biomass and oleic acid (C18:1) was catalyzed by immobilized-stabilized derivatives of Candida antarctica lipase B in a methyl ethyl ketone medium. After steam-explosion pretreatment of mixed hardwoods and enzymatic hydrolysis at 15%wt solids, xylose and glucose were purified/concentrated to a mass ratio of similar to 3 to 1. These lignocellulosic sugars were superior to commercial sugars as the carbohydrate source for the esterification reaction in terms of sugar conversions. The highest conversions were obtained using 1.5% w/v of Novozyme 435 (N435, uncoated) as the biocatalyst for the synthesis of SFAEs. Coating the N435 with polyethyleneimine (PEI) prevented enzyme leakage into the reaction medium and produced 35% and 50% higher xylose and glucose conversions to SFAEs, respectively, at the same enzyme loading. After six 24 h reuse cycles with the PEI-coated N435, xylose conversion decreased by 44%, while a 65% reduction in xylose conversion was observed with the uncoated lipase. Mass spectrometry analysis confirmed the production of xylose and glucose mono- and di-esters. Our purified product presented an emulsion capacity (EC) close to that of a commercial sugar ester and the ECs of the xylose oleate, laurate, and palmitate synthesized in previous studies. (c) 2023 The Authors. Biofuels, Bioproducts and Biorefining published by Society of Industrial Chemistry and John Wiley & Sons Ltd. (AU)

• Processo FAPESP: 21/06525-4 - Produção e purificação de açúcares mistos para uso na síntese de ésteres graxos de xilose catalisados por derivados estabilizados imobilizados de Candida antarctica lipase B
• Beneficiário: Maria Carolina Pereira Gonçalves
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 19/23908-4 - Design de novos biocatalisadores por modificações de superfície de lipases pós-imobilização para aplicação na síntese de oleato de xilose
• Beneficiário: Maria Carolina Pereira Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático