Identification of miRnas with possible prognostic roles for HAM/TSP

Autor(es)

de Souza, Daniela Raguer Valadao ; Pessoa, Rodrigo ; Nukui, Youko ; Pereira, Juliana ; Marcusso, Rosa Nascimento ; de Oliveira, Augusto Cesar Penalva ; Casseb, Jorge ; Duarte, Alberto Jose da Silva ; Clissa, Patricia Bianca ; Sanabani, Sabri Saeed

• Tipo de documento: Artigo Científico
• Fonte: VIRULENCE; v. 14, n. 1, p. 16-pg., 2023-12-31.

Resumo

Human T-cell lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropic spastic paraparesis (HAM/TSP) is an insidiously progressive spinal cord disease for which there is no effective treatment. There is great interest in developing potential biomarkers to predict the pathogenesis of HAM/TSP disease. In this study, Illumina Massive Parallel Sequencing (MPS) technology was used to investigate the cellular global noncoding RNAome expression profile in HAM/TSP patients (n = 10), asymptomatic HTLV-1-infected carriers (ASP, n = 8), and a second group of healthy controls (n = 5). Various bioinformatics tools were used to align, annotate, and profile the sRNA-MPS reads. Among the 402 sRNAs detected, 251 were known and 50 were potentially novel sRNAs in the HAM and ASP groups compared with the HC group. Sixty-eight known sRNAs were significantly different between the ASP and HAM groups. Eighty-eight mature miRNAs were downregulated in subjects from HAM compared with ASP. Three of these miRs (hsa-miR-185-5p, 32-5p, and 192-5p) have the potential to be used as biomarkers for predicting the pathogenesis of HAM/TSP. The seven most deregulated miRs target genes have been associated with a variety of biological processes and molecular functions. The reactome pathways relevant to our findings provide a rich source of data and offer the opportunity to better understand sRNA regulation and function in HTLV-1 pathophysiology. To the best of our knowledge, this study is the first to demonstrate evaluates sRNAs in HTLV-1 patients with HAM/TSP. (AU)

• Processo FAPESP: 22/09354-9 - Perfil do metaboloma e do bacterioma em pacientes HAM/TSP
• Beneficiário: Sabri Saeed Mohammed Ahmed Al-Sanabani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/12297-2 - Análise dos transcritos de miRNAs, REX e tax em células T no curso da infecção pelo HTLV-1, utilizando sequenciamento de nova geração
• Beneficiário: Sabri Saeed Mohammed Ahmed Al-Sanabani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/24596-2 - Sequenciamento em larga escala dos genomas do HIV na forma de RNA livre e DNA integrado: comparação entre os subtipos gerados, mutações relacionadas a resistência de drogas e o uso de co-receptores
• Beneficiário: Rodrigo Pessoa de Farias
• Modalidade de apoio: Bolsas no Brasil - Doutorado