| Texto completo | |
| Autor(es): |
Bem, Jessica Silva Peixoto
;
Lacerda, Nayanna Gomes Silva
;
Polizello, Ana Cristina Morseli
;
Cabral, Hamilton
;
da Rosa-Garzon, Nathalia Gonsales
;
Aires, Carolina Patricia
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Current Microbiology; v. 80, n. 9, p. 10-pg., 2023-09-01. |
| Resumo | |
This study aimed to evaluate the disruptive effect of fungal mutanase against cariogenic biofilm after short-term treatment. For that, mature Streptococcus mutans biofilms (n = 9) were exposed to active or inactivated enzymes produced by Trichoderma harzianum for 1 min, two times per day. Biofilms were analyzed by amount of matrix water-insoluble polysaccharides, bacterial viability, acidogenicity, and morphology by scanning electron microscopy (SEM). The group treated with active enzymes (AE) had a significantly lower amount of insoluble polysaccharides (893.30 & PLUSMN; 293.69) when compared to the negative control group (NaCl, 2192.59 & PLUSMN; 361.96), yet no significant difference was found when comparing to the positive control group (CHX, 436.82 & PLUSMN; 151.07). Also, there was no significant effect on bacteria metabolism and viability (P-value < 0.05). Data generated by the quantitative analysis were confirmed through scanning electron microscopy images. Thus, fungal mutanase degraded the biofilm after a short-term treatment without interfering with bacterial viability and metabolism. Such findings offer insight to the development of routine oral care products containing this input. (AU) | |
| Processo FAPESP: | 19/19162-7 - O desafio do controle de biofilme em pacientes com uso de disjuntor palatino: efeito de enzimas produzidas pelo fungo Trichoderma harzianum |
| Beneficiário: | Nayanna Gomes Silva Lacerda |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 20/07315-0 - Resíduos industriais de manga para controle de biofilme cariogênico: estudo in vitro |
| Beneficiário: | Jéssica Silva Peixoto Bem |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |