Quantitative proteomic profile analysis of thyroid dysfunction effects on seminal vesicles and repercussions on male fertility

Autor(es)

Correa, Deborah Elzita do Carmo ; Bargi-Souza, Paula ; Oliveira, Isabela Medeiros ; Razera, Amanda ; Oliveira, Claudio Alvarenga ; Romano, Marco Aurelio ; Romano, Renata Marino

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Cellular Endocrinology; v. 578, p. 15-pg., 2023-12-01.

Resumo

Hypothyroidism and thyrotoxicosis are associated with male reproductive disorders, but little is known about the influence of the thyroid hormone milieu on seminal vesicle (SV) function and metabolism. In this sense, we investigated the effects of hypothyroidism and thyrotoxicosis induced in adulthood Wistar male rats on SV function and identified new thyroid hormone targets on male reproduction regulation using novel proteomic approaches. Hypothyroidism reduces SV size and seminal fluid volume, which are directly associated with low testosterone and estradiol levels, while thyrotoxicosis increases Esr2 and Dio1 expression in the SV. We found 116 differentially expressed proteins. Hypothyroidism reduces the expression of molecular protein markers related to sperm viability, capacitation and fertilization, protection against oxidative stress and energetic metabolism in SV, while it increases the expression of proteins related to tissue damage. In conclusion, thyroid dysfunction in the adult phase impairs several morphological, molecular and functional characteristics of SV. (AU)

• Processo FAPESP: 14/10845-0 - Consequências do hipotireoidismo e da tirotoxicose desenvolvidos na vida adulta sobre a função gonadal masculina: expressão gênica de marcadores moleculares e perfil proteômico em tecidos do eixo hipotalâmico-hipofisário-gonadal
• Beneficiário: Claudio Alvarenga de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular