Where do you measure the Bregma for rodent stereotaxic surgery?

Autor(es)

Cecyn, Marianna Nogueira ; Abrahao, Karina Possa

• Tipo de documento: Artigo Científico
• Fonte: IBRO NEUROSCIENCE REPORTS; v. 15, p. 6-pg., 2023-12-01.

Resumo

The advent of the stereotaxic apparatus developed by Clarke and Horsley revolutionized neuroscience research, enabling precise 3D navigation along the skull mediolateral, anteroposterior, and dorsoventral axes. In rodents, the Bregma is widely used as the origin reference point for the stereotaxic coordinates, but the specific procedure for its measurement varies among different laboratories. Notably, the renowned brain atlas developed by Paxinos and Franklin lacks explicit instructions on the Bregma determination. Recent studies have found discrepancies in skull and brain landmark measurements. This review describes the commonly used brain atlases and highlights the limitations in accurately measuring the stereotaxic coordinates. In addition, we propose alternative and more reliable approaches to measure the Bregma. It is imperative to address the misconceptions about the accuracy of stereotaxic surgeries, as it can significantly impact a substantial portion of neuroscience research. (AU)

• Processo FAPESP: 19/01686-0 - Depois do primeiro ‘drink’, não consigo parar! O efeito do álcool nos sistemas cerebrais de controle inibitório
• Beneficiário: Karina Possa Abrahão
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores