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From enzyme to cell-factory: Economic and environmental assessment of biobased pathways to unlock the potential of long-haul transportation biofuels

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Autor(es):
Chagas, Mateus Ferreira ; Ferreira, Gabriela Filipini ; Gomes, Luisa Clemente Magalhaes ; Zanphorlin, Leticia Maria ; Martins, Liliana Zanelli Oliveira ; Sampaio, Isabelle Lobo Mesquita ; Hernandes, Thayse Aparecida Dourado ; Junqueira, Tassia Lopes ; Morais, Edvaldo Rodrigo
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: CHEMICAL ENGINEERING JOURNAL; v. 469, p. 16-pg., 2023-06-09.
Resumo

The transportation sector significantly contributes to greenhouse gas emissions due to its reliance on fossil fuels. Biofuels offer a low-carbon alternative, particularly for long-haul transportation. Although few biosynthetic pathways reached technological maturity with commercial plants, recent literature shows a variety of laboratoryscale progresses targeting these biofuels. Early-stage sustainability assessments can help identify opportunities for metabolic engineering and process improvements toward more sustainable routes. This study evaluated the technical, economic, and environmental aspects of producing drop-in biofuels from sugarcane and soybean oil, considering fermentative and enzymatic processes. The results showed that sugarcane-based scenarios presented a better prospect and that integrating steps within microorganisms' metabolism in a cell factory concept had advantages over the cell-free enzymatic process. Biobased pathways, however, still require improvements to be competitive with catalytic-based alternatives. Recommendations were made to guide microorganism engineering, focusing on factors that restrain economic and environmental feasibility (e.g., inputs use). As a result, the improved biobased process could provide a hydrocarbon-based fuel with a minimum selling price (US$ 21.2 GJ-1) compatible with market prices and carbon intensity (25.1 gCO2eq MJ-1) similar to that from sugarcane ethanol, representing over 70 % reduction in greenhouse gas (GHG) emissions compared to fossil fuels. (AU)

Processo FAPESP: 19/08855-1 - Novos mecanismos de P450: uma estratégia enzimática para a produção de hidrocarbonetos renováveis
Beneficiário:Leticia Maria Zanphorlin
Modalidade de apoio: Auxílio à Pesquisa - Regular