| Texto completo | |
| Autor(es): |
Flores Abuna, Rodrigo Paolo
;
Oliveira, Fabiola Singaretti
;
Adolpho, Leticia Faustino
;
Fernandes, Roger Rodrigo
;
Rosa, Adalberto Luiz
;
Beloti, Marcio Mateus
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Cellular Physiology; v. 235, n. 11, p. 11-pg., 2020-04-02. |
| Resumo | |
This study aimed to investigate if wingless-related integration site (Wnt) signaling participates in the high osteogenic potential of titanium with nanotopography (Ti-Nano). We showed that among the several components of the Wnt signaling pathway, Frizzled 6 (Fzd6) was the transcript most intensely modulated by nanotopography compared with the untreated Ti surface (Ti-Machined). Then, we investigated whether and how Fzd6 participates in the regulation of osteoblast differentiation caused by nanotopography. The Fzd6 silencing with CRISPR-Cas9 transfection in MC3T3-E1 cells induced a more pronounced inhibition of osteoblast differentiation of cells cultured on nanotopography than those cultured on Ti-Machined. The analysis of the expression of calcium-calmodulin-dependent protein kinase II and beta-catenin demonstrated that Fzd6 disruption inhibited the osteoblast differentiation induced by Ti-Nano by preventing the activation of Wnt/beta-catenin but not that of Wnt/Ca2+ signaling, which is usually triggered by the receptor Fzd6. These findings elucidate the biological function of Fzd6 as a receptor that triggers Wnt/beta-catenin signaling and the cellular mechanisms modulated by nanotopography during osteoblast differentiation. (AU) | |
| Processo FAPESP: | 16/14171-0 - Papel das Vias de Sinalização de Wnt no Potencial Osteogênico de Superfície de Titânio com Nanotopografia |
| Beneficiário: | Márcio Mateus Beloti |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/17356-6 - Efeito da Superfície de Titânio com Nanotopografia sobre a Interação entre Osteoblastos e Osteoclastos |
| Beneficiário: | Márcio Mateus Beloti |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 16/14711-4 - Papel das Vias de Sinalização de Wnt no Potencial Osteogênico de Superfície de Titânio com Nanotopografia |
| Beneficiário: | Rodrigo Paolo Flores Abuna |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |