Busca avançada
Ano de início
Entree


Model Membranes and Antimicrobial Activities of pH-Sensitive Copolymers

Texto completo
Autor(es):
Mostrar menos -
Saraiva, Greice K. ; de Souza, Valdomiro V. ; de Oliveira, Luciana C. ; Procopio, Alyne ; Martins, Maisa T. ; Aidar, Isabel N. ; Yi, Ronaldo C. F. ; Lacerda, Caroline D. ; Carretero, Gustavo P. B. ; de Lira, Rafael B. ; Riske, Karin A. ; Salinas, Roberto K. ; Chaimovich, Hernan ; Florenzano, Fabio H. ; Cuccovia, Iolanda M.
Número total de Autores: 15
Tipo de documento: Artigo Científico
Fonte: Journal of the Brazilian Chemical Society; v. N/A, p. 14-pg., 2023-05-22.
Resumo

Polymers are options as antimicrobials for skin protection, antifouling surfaces, and fabrics. Here we analyzed the interaction of polymers based on poly(methacrylate) (PMMA) and poly((dimethylamino ethyl) methacrylate) (PDMAEMA) with model membranes and bacteria. We used the homopolymers PMMA, PDMAEMA, and the diblock copolymer(s) prepared with different PMMAm:PDMAEMAn ratios (m/n). The interactions of PDMAEMA and PMMAm-b-PDMAEMAn with large unilamellar vesicles (LUVs) prepared with phosphatidylcholine and phosphatidylglycerol at different pHs, were analyzed by nuclear magnetic resonance (NMR), dynamic light scattering, and zeta potential. These polymers promoted LUVs leakage of a fluorescent probe (5,6-carboxyfluorescein) localized exclusively in the internal aqueous compartment. Interestingly, all copolymers exhibit a bell-shaped pH dependence for the polymer-induced LUVs leakage. The interaction of the positively charged polymers and the pH effect was also demonstrated using giant unilamellar vesicles. These copolymers inhibited bacterial growth in the micromolar range and can be used to prevent bacterial growth on surfaces. (AU)

Processo FAPESP: 18/15230-5 - Ação antibacteriana e anticâncer do peptídeo BP100 e seus derivados contendo Naftalimida
Beneficiário:Gustavo Penteado Battesini Carretero
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/08166-5 - Química em interfaces: interações de fármacos, peptídios e enzimas com membranas modelos
Beneficiário:Iolanda Midea Cuccovia
Modalidade de apoio: Auxílio à Pesquisa - Temático