Titanium biogenic nanoparticles to help the growth of Trichoderma harzianum to be used in biological control

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Pasquoto-Stigliani, Tatiane ; Guilger-Casagrande, Mariana ; Campos, Estefania V. R. ; Germano-Costa, Tais ; Bilesky-Jose, Natalia ; Migliorini, Bianca B. ; Feitosa, Leandro O. ; Sousa, Bruno T. ; de Oliveira, Halley C. ; Fraceto, Leonardo F. ; Lima, Renata

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NANOBIOTECHNOLOGY; v. 21, n. 1, p. 21-pg., 2023-05-25.

Resumo

Background The biogenic synthesis of metallic nanoparticles is a green alternative that reduces the toxicity of this nanomaterials and may enable a synergy between the metallic core and the biomolecules employed in the process enhancing biological activity. The aim of this study was to synthesize biogenic titanium nanoparticles using the filtrate of the fungus Trichoderma harzianum as a stabilizing agent, to obtain a potential biological activity against phytopathogens and mainly stimulate the growth of T. harzianum, enhancing its efficacy for biological control.Results The synthesis was successful and reproductive structures remained in the suspension, showing faster and larger mycelial growth compared to commercial T. harzianum and filtrate. The nanoparticles with residual T. harzianum growth showed inhibitory potential against Sclerotinia sclerotiorum mycelial growth and the formation of new resistant structures. A great chitinolytic activity of the nanoparticles was observed in comparison with T. harzianum. In regard to toxicity evaluation, an absence of cytotoxicity and a protective effect of the nanoparticles was observed through MTT and Trypan blue assay. No genotoxicity was observed on V79-4 and 3T3 cell lines while HaCat showed higher sensitivity. Microorganisms of agricultural importance were not affected by the exposure to the nanoparticles, however a decrease in the number of nitrogen cycling bacteria was observed. In regard to phytotoxicity, the nanoparticles did not cause morphological and biochemical changes on soybean plants.Conclusion The production of biogenic nanoparticles was an essential factor in stimulating or maintaining structures that are important for biological control, showing that this may be an essential strategy to stimulate the growth of biocontrol organisms to promote more sustainable agriculture. (AU)

• Processo FAPESP: 16/13692-6 - Nanopartículas biogênicas de prata baseadas em Trichoderma harzianum: diferentes condições de síntese, avaliação da cito/genotoxicidade e potencial inibitório de Sclerotinia sclerotiorum
• Beneficiário: Mariana Guilger Casagrande
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 20/05816-2 - Estudo do recobrimento (capping) de nanopartículas biogênicas e sua correlação com toxicidade e atividade no controle de fitopatógenos
• Beneficiário: Mariana Guilger Casagrande
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/13328-5 - Desenvolvimento e avaliação de nanopartiulas metálicas biogênicas (prata, titânio e ferro) baseadas em Trichoderma harzianum para aplicação agrícola
• Beneficiário: Renata de Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/21004-5 - Agricultura, micro/nanotecnologia e ambiente: da avaliação dos mecanismos de ação a estudos de transporte e toxicidade
• Beneficiário: Leonardo Fernandes Fraceto
• Modalidade de apoio: Auxílio à Pesquisa - Temático