Proteomic characterization of metal recovery process realized by marine bacteria bacillus subtilis Hyhel1expossed to bioleaching liquor

Autor(es)

Rozas, Enrique E. E. ; Dias, Meriellen ; Acosta, Arlen Mabel Lastre ; Custodio, Marcio Reis ; do Nascimento, Claudio A. O. ; Mendes, Maria Anita

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Chemical Engineering; v. N/A, p. 10-pg., 2023-05-30.

Resumo

Metal transporter proteins (MTP) observed in Hyhel1 enabled selective recovering of metal present in bioleaching liquor (BL). Changes in the MTP profiles suggest that bacteria produced specific proteins in response to BL, allowing metal recovery. To characterize proteomic uptake of metal by Hyhel, bacteria were incubated in BL at 30 degrees C for 24 h at 150 RPM. After treatment, bacteria were centrifuged, lyophilized, and had its elemental composition determined by DRX. Bacteria proteins were then extracted, followed by trypsin digestion, mass spectrometry analysis and data submission to Mascot software for protein identification. Differences between control and treated BL were established by protein comparison using a Venn diagram and gene ontology analysis. DRX showed metal bioaccumulation of Fe, Cu, Al, Cr and Ni, with higher concentrations of Fe and Cu. Changes in type and number of MTP were determined by proteomic analysis in treated bacteria BL. The results suggest that copper was transported inside bacteria through Omp (A, B, C and X) and ABCph MTP, whereas iron was transported by FepA and ABCth, allowing bioaccumulation of both metals. Thus, proteomic analysis determined that Hyhel1 when adapted to BL modifies the composition of MTP membranes enabling iron and copper recovery in the acidic environment. (AU)

• Processo FAPESP: 13/50218-2 - CEPEMA - Centro Cooperativo em Engenharia Ambiental
• Beneficiário: Claudio Augusto Oller do Nascimento
• Modalidade de apoio: Auxílio à Pesquisa - Temático