Aspergillus fumigatus Extracellular Vesicles Display Increased Galleria mellonella Survival but Partial Pro-Inflammatory Response by Macrophages

Autor(es)

Freitas, Mateus Silveira ; Bitencourt, Tamires Aparecida ; Rezende, Caroline Patini ; Martins, Nubia Sabrina ; Dourado, Thales de Mileto Henrique ; Tirapelli, Carlos R. ; Almeida, Fausto

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF FUNGI; v. 9, n. 5, p. 15-pg., 2023-05-04.

Resumo

Fungal extracellular vesicles (EVs) mediate intra- and interspecies communication and are critical in host-fungus interaction, modulating inflammation and immune responses. In this study, we evaluated the in vitro pro- and anti-inflammatory properties of Aspergillus fumigatus EVs over innate leukocytes. A. fumigatus EVs induced a partial proinflammatory response by macrophages, characterized by increased tumor necrosis factor-alpha production, and increased gene expression of induced nitric oxide synthase and adhesion molecules. EVs induce neither NETosis in human neutrophils nor cytokine secretion by peripheral mononuclear cells. However, prior inoculation of A. fumigatus EVs in Galleria mellonella larvae resulted in increased survival after the fungal challenge. Taken together, these findings show that A. fumigatus EVs play a role in protection against fungal infection, although they induce a partial pro-inflammatory response. (AU)

• Processo FAPESP: 21/06794-5 - Vesículas extracelulares fúngicas: imunomodulação e comunicação celular
• Beneficiário: Fausto Bruno dos Reis Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 19/22454-0 - Avaliação do potencial imunomodulador de vesículas extracelulares produzidas por Aspergillus fumigatus
• Beneficiário: Mateus Silveira Freitas
• Modalidade de apoio: Bolsas no Brasil - Doutorado