Contrasting effects of transdermal and implant corticosterone treatments in the American bullfrog wound healing

Autor(es)

Madelaire, Carla B. ; Silva, Diego P. ; Titon, Stefanny C. M. ; Lamadrid-Feris, Faride ; Floreste, Felipe R. ; Titon Jr, Braz ; Gomes, Fernando R.

• Tipo de documento: Artigo Científico
• Fonte: PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES; v. 378, n. 1882, p. 11-pg., 2023-07-31.

Resumo

Glucocorticoid (GC) release is triggered by adverse stimuli that activate the hypothalamus-pituitary-adrenal/interrenal axis. Glucocorticoids may enhance or suppress immune functions depending on the level of elevation. In this study, we investigated the effects of transient and chronic increase of corticosterone (CORT) on the wound healing of the American bullfrog. Frogs were submitted to a daily transdermal hormonal application that acutely elevated CORT plasma levels, or vehicle as a control. Other frogs were surgically implanted with a silastic tube filled with CORT that resulted in chronic elevation of CORT plasma levels or received empty implants as a control. A dermal biopsy was performed to create a wound and was photographed every 3 days. Individuals treated with transdermal CORT started healing faster than their control 32 days after the biopsy. Frogs that received CORT implants tended to heal slower than control subjects. Plasma bacterial killing ability was not affected by treatment, which reinforces the constitutive nature of this innate immune trait. By the end of the experiment, frogs from the acute CORT treatment had smaller wounds compared with those receiving the CORT-filled implants, highlighting the differential effects of acute (immunoenhancing) and chronic (immunosuppressive) elevation of CORT plasma levels.This article is part of the theme issue 'Amphibian immunity: stress, disease and ecoimmunology'. (AU)

• Processo FAPESP: 14/16320-7 - Impactos das mudanças climáticas e ambientais sobre a fauna: uma abordagem integrativa
• Beneficiário: Carlos Arturo Navas Iannini
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático
• Processo FAPESP: 18/00406-0 - Relação sazonal entre imunidade, hormônios esteroides e resposta ao estresse em modelos ectotermos
• Beneficiário: Carla Bonetti Madelaire
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado