Streptomyces hilarionis sp. nov. and Streptomyces hayashii sp. nov., two new strains associated with potato scab in Brazil

Autor(es)

Vitor, Lucas ; Amaral, Danilo Trabuco ; Correa, Daniele Bussioli Alves ; Ferreira-Tonin, Mariana ; Lucon, Emanuel Torres ; Appy, Mariana Pereira ; Tomaseto, Alex Augusto ; Destefano, Suzete Aparecida Lanza

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY; v. 73, n. 6, p. 11-pg., 2023-01-01.

Resumo

Two new actinobacteria, designated strains IBSBF 2807T and IBSBF 2953T, isolated from scab lesions on potato tubers grown in the southern Brazilian states of Rio Grande do Sul and Santa Catarina, respectively, were characterized and iden-tified through a polyphasic approach. Phylogenetic analyses of 16S rRNA sequences revealed that these two strains belong to the genus Streptomyces. Multilocus sequence analysis using five concatenated genes, atpD, gyrB, recA, rpoB and trpB, allocated strains IBSBF 2807T and IBSBF 2953T in distinct branches of Streptomyces phytopathogenic strains. PCR- RFLP analysis of the atpD gene also confirmed that these strains differ from the type strains of Streptomyces associated with potato scab. The morphological, physiological and biochemical characterization, along with the overall genome-related index properties, indicated that these two strains could be distinguished from their closest phylogenetic relatives and each other. According to the data, IBSBF 2807T and IBSBF 2953T represent two new Streptomyces species related to potato scab. The proposed names for these strains are Streptomyces hilarionis sp. nov. (IBSBF 2807T=CBMAI 2674T=ICMP 24297T=MUM 22.66T) and Streptomyces hayashii sp. nov (IBSBF 2953T=CBMAI 2675T=ICMP 24301T=MUM 22.68T). (AU)

• Processo FAPESP: 17/50334-3 - Plano de Desenvolvimento Institucional em Pesquisa (PDIp): modernização e adequação de unidades multiusuárias estratégicas do Instituto Biológico
• Beneficiário: Ana Eugênia de Carvalho Campos
• Modalidade de apoio: Auxílio à Pesquisa - Programa Modernização de Institutos Estaduais de Pesquisa
• Processo FAPESP: 19/12999-9 - Identificação de novas espécies de Streptomyces associadas a sarna da batata
• Beneficiário: Lucas Vitor
• Modalidade de apoio: Bolsas no Brasil - Mestrado