Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes

Autor(es):Mostrar menos -

Miranda, Luis Fernando B. ; Lima, Carolina V. ; Pagin, Rafaela ; Costa, Raphael C. ; Pereira, Marta Maria A. ; de Avila, Erica D. ; Bertolini, Martinna ; Retamal-Valdes, Belen ; Shibli, Jamil A. ; Feres, Magda ; Barao, Valentim A. R. ; Souza, Joao Gabriel S.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOME RESEARCH; v. 22, n. 3, p. 14-pg., 2023-03-03.

Resumo

The use of saliva as a protein source prior to microbiological and biological assays requires previous processing. However, the effect of these processing methods on the proteomic profile of saliva has not been tested. Stimulated human saliva was collected from eight healthy volunteers. Non-processed saliva was compared with 0.22 mu m filtered, 0.45 mu m filtered, and pasteurized saliva, by liquid chromatography-mass spectrometry. Data are available via ProteomeXchange with identifier PXD039248. The effect of processed saliva on microbial adhesion was tested using bacterial and fungus species and in biological cell behavior using HaCaT immortalized human keratinocytes. Two hundred and seventy-eight proteins were identified in non-processed saliva, of which 54 proteins (approximate to 19%) were exclusive. Saliva processing reduced identified proteins to 222 (approximate to 80%) for the 0.22 mu m group, 219 (approximate to 79%) for the 0.45 mu m group, and 201 (approximate to 72%) for the pasteurized saliva, compared to non-processed saliva. The proteomic profile showed similar molecular functions and biological processes. The different saliva processing methods did not alter microbial adhesion (ANOVA, p > 0.05). Interestingly, pasteurized saliva reduced keratinocyte cell viability. Saliva processing methods tested reduced the proteomic profile diversity of saliva but maintained similar molecular functions and biological processes, not interfering with microbial adhesion and cell viability, except for pasteurization, which reduced cell viability. (AU)

• Processo FAPESP: 21/09434-0 - Um novo revestimento antimicrobiano para liberação de droga direcionado a dispositivos implantares percutâneos
• Beneficiário: Érica Dorigatti de Avila
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 18/20719-3 - Um novo revestimento antimicrobiano para liberação de droga direcionado a dispositivos implantares percutâneos
• Beneficiário: Érica Dorigatti de Avila
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 20/10436-4 - Síntese, caracterização e desempenho biológico de nanopolímeros pH-sensitivos produzidos por impressão molecular para o tratamento das infecções peri-implantares
• Beneficiário: Raphael Cavalcante Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 21/10762-1 - Desenvolvimento de filme de material regenerativo vascular baseado em MAA sobre revestimento antimicrobiano de [PAA/PLL]10/TC/aniônico B-CD para dispositivos de implantes percutâneos
• Beneficiário: Marta Maria Alves Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto