The potential of using Metarhizium anisopliae and Metarhizium humberi to control the Asian soybean rust caused by Phakopsora pachyrhizi

Autor(es)

Holz, Sabrina ; D'Alessandro, Celeste Paola ; Maximo, Heros Jose ; de Souza, Paulo Henrique Nascimento ; Raruang, Yenjit ; Demetrio, Clarice Garcia Borges ; Delalibera Junior, Italo ; Chen, Zhi-Yuan ; Pascholati, Sergio Florentino

• Tipo de documento: Artigo Científico
• Fonte: BIOCONTROL SCIENCE AND TECHNOLOGY; v. 33, n. 4, p. 17-pg., 2023-03-21.

Resumo

Phakopsora pachyrhizi causes Asian soybean rust, resulting in severe losses of soybean yield. This study assessed the biocontrol efficacy of cell-free culture filtrates and conidial suspensions of Metarhizium anisopliae (MABR-01) and M. humberi (MHBR-03) against P. pachyrhizi in vitro and in planta. A 50% concentration of culture filtrates of M. anisopliae and M. humberi inhibited the germination of P. pachyrhizi urediniospores by 85 and 96%, respectively, compared to the 50% potato-dextrose broth (PDB) control. The conidial suspensions caused no inhibition of P. pachyrhizi in vitro, but the conidial suspension of M. anisopliae controlled the rust disease in plants with a 51% efficiency. Soybean plants colonised by M. anisopliae MABR-01 and exposed to P. pachyrhizi showed less severe disease in the shoots compared to plants not colonised by M. anisopliae; the disease was reduced by 40%. Spraying plants with 50 and 75% culture filtrates of M. humberi and a 75% concentration of M. anisopliae significantly reduced rust disease, by an average of 86%. Plants pretreated with Metarhizium culture filtrates showed a 30% reduction in P. pachyrhizi colonisation based on qPCR quantification of the pathogen genomic DNA. These results indicate a high biotechnological and sustainable potential of these two Metarhizium species for control of Asian soybean rust. (AU)

• Processo FAPESP: 18/18393-2 - Potencial de diferentes isolados de Metarhizium sp no controle alternativo (indução de resistência) em soja a Phakopsora pachyrhizi
• Beneficiário: Sabrina Holz
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 19/23030-9 - Desenvolvimento de produto biotecnológico contendo o fungo Memnoniella levispora para uso na agricultura
• Beneficiário: Héros José Maximo
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE
• Processo FAPESP: 19/19767-6 - Determinar o(s) ingrediente(s) ativo(s) no filtrado de Metarhizium sp. com atividade anti-ferrugem e explorar o potencial da aplicação direta de dsRNA para manejar a doença da ferrugem da soja
• Beneficiário: Sabrina Holz
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado