| Texto completo | |
| Autor(es): |
Holz, Sabrina
;
D'Alessandro, Celeste Paola
;
Maximo, Heros Jose
;
de Souza, Paulo Henrique Nascimento
;
Raruang, Yenjit
;
Demetrio, Clarice Garcia Borges
;
Delalibera Junior, Italo
;
Chen, Zhi-Yuan
;
Pascholati, Sergio Florentino
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOCONTROL SCIENCE AND TECHNOLOGY; v. 33, n. 4, p. 17-pg., 2023-03-21. |
| Resumo | |
Phakopsora pachyrhizi causes Asian soybean rust, resulting in severe losses of soybean yield. This study assessed the biocontrol efficacy of cell-free culture filtrates and conidial suspensions of Metarhizium anisopliae (MABR-01) and M. humberi (MHBR-03) against P. pachyrhizi in vitro and in planta. A 50% concentration of culture filtrates of M. anisopliae and M. humberi inhibited the germination of P. pachyrhizi urediniospores by 85 and 96%, respectively, compared to the 50% potato-dextrose broth (PDB) control. The conidial suspensions caused no inhibition of P. pachyrhizi in vitro, but the conidial suspension of M. anisopliae controlled the rust disease in plants with a 51% efficiency. Soybean plants colonised by M. anisopliae MABR-01 and exposed to P. pachyrhizi showed less severe disease in the shoots compared to plants not colonised by M. anisopliae; the disease was reduced by 40%. Spraying plants with 50 and 75% culture filtrates of M. humberi and a 75% concentration of M. anisopliae significantly reduced rust disease, by an average of 86%. Plants pretreated with Metarhizium culture filtrates showed a 30% reduction in P. pachyrhizi colonisation based on qPCR quantification of the pathogen genomic DNA. These results indicate a high biotechnological and sustainable potential of these two Metarhizium species for control of Asian soybean rust. (AU) | |
| Processo FAPESP: | 18/18393-2 - Potencial de diferentes isolados de Metarhizium sp no controle alternativo (indução de resistência) em soja a Phakopsora pachyrhizi |
| Beneficiário: | Sabrina Holz |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 19/23030-9 - Desenvolvimento de produto biotecnológico contendo o fungo Memnoniella levispora para uso na agricultura |
| Beneficiário: | Héros José Maximo |
| Modalidade de apoio: | Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE |
| Processo FAPESP: | 19/19767-6 - Determinar o(s) ingrediente(s) ativo(s) no filtrado de Metarhizium sp. com atividade anti-ferrugem e explorar o potencial da aplicação direta de dsRNA para manejar a doença da ferrugem da soja |
| Beneficiário: | Sabrina Holz |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Mestrado |