Photoelectrochemical Determination of Cardiac Troponin I as a Biomarker of Myocardial Infarction Using a Bi2S3 Film Electrodeposited on a BiVO4-Coated Fluorine-Doped Tin Oxide Electrode

Autor(es)

Monteiro, Thatyara Oliveira ; Neto, Antonio Gomes dos Santos ; de Menezes, Alan Silva ; Damos, Flavio Santos ; Luz, Rita de Cassia Silva ; Fatibello-Filho, Orlando

• Tipo de documento: Artigo Científico
• Fonte: BIOSENSORS-BASEL; v. 13, n. 3, p. 15-pg., 2023-03-01.

Resumo

A sensitive and selective label-free photoelectrochemical (PEC) immunosensor was designed for the detection of cardiac troponin I (cTnI). The platform was based on a fluorine-doped tin oxide (FTO)-coated glass photoelectrode modified with bismuth vanadate (BiVO4) and sensitized by an electrodeposited bismuth sulfide (Bi2S3) film. The PEC response of the Bi2S3/BiVO4/FTO platform for the ascorbic acid (AA) donor molecule was approximately 1.6-fold higher than the response observed in the absence of Bi2S3. The cTnI antibodies (anti-cTnI) were immobilized on the Bi2S3/BiVO4/FTO platform surface to produce the anti-cTnI/Bi2S3/BiVO4/FTO immunosensor, which was incubated in cTnI solution to inhibit the AA photocurrent. The photocurrent obtained by the proposed immunosensor presented a linear relationship with the logarithm of the cTnI concentration, ranging from 1 pg mL(-1) to 1000 ng mL(-1). The immunosensor was successfully employed in artificial blood plasma samples for the detection of cTnI, with recovery values ranging from 98.0% to 98.5%. (AU)

• Processo FAPESP: 20/01050-5 - Desenvolvimento e aplicação de sensores eletroquímicos e fotoeletroquímicos para a determinação de contaminantes emergentes em amostras de águas naturais
• Beneficiário: Orlando Fatibello Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular