| Autor(es): |
Kim, Yeon Jung
;
Viana, Aline Cavalcanti
;
Tfaile Corbi, Samia Cruz
;
de Carvalho Curtis, Karen Maria
;
Renzi, Rivelto
;
Perez Orrico, Silvana Regina
;
Cirelli, Joni Augusto
;
Scarel-Caminaga, Raquel Mantuaneli
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOSCIENCE JOURNAL; v. 25, n. 6, p. 7-pg., 2009-11-01. |
| Resumo | |
Interleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms ( SNPs) in the human IL8 gene have been investigated in many disease association studies. We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene. This method was used for typing 147 white healthy Brazilian individuals, whose DNA was obtained from buccal epithelial cells and extracted with phenol: chloroform: isoamyl alcohol. Genomic DNA was amplified by PCR using a conventional thermal cycler. The PCR products ( 573 bp) were submitted to RFLP reactions. The RFLP fragments were analyzed in a 4% agarose gel stained with ethidium bromide. The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other white populations in the SNP Database of the National Center for Biotechnology Information (NCBI). Because the PCR-RFLP method presented here was efficient, low cost, reproducible and convenient for laboratories with a limited level of technology worldwide, it should be useful for genotyping in case-control association or population genetic studies. (AU) | |
| Processo FAPESP: | 05/04553-8 - Investigação de polimorfismos no gene da interleucina 10 em indivíduos com periodontite agressiva |
| Beneficiário: | Patrícia Mayumi Sogumo |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 05/03231-7 - Investigação de polimorfismos no promotor do gene interleucina 8 em indivíduos com doença periodontal crônica |
| Beneficiário: | Yeon Jung Kim |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 06/04492-1 - Investigacao de polimorfismos no gene interleucina 8 e seus receptores em individuos com doenca periodontal cronica. |
| Beneficiário: | Karen Maria de Carvalho Curtis |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 03/10424-0 - Investigação de polimorfismos no gene interleucina 8 e seus receptores em indivíduos com doença periodontal crônica |
| Beneficiário: | Raquel Mantuaneli Scarel Caminaga |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |