Nanoparticles of Molybdenum Chlorophyllin Photosensitizer and Magnetic Citrate-Coated Cobalt Ferrite Complex Available to Hyperthermia and Photodynamic Therapy Clinical Trials

Autor(es)

Primo, Fernando L. ; Cordo, Paloma L. A. G. ; Neto, Alberto F. ; Morais, Paulo C. ; Tedesco, Antonio C. ; Hafeli, U ; Schutt, W ; Zborowski, M

• Tipo de documento: Artigo Científico
• Fonte: MAGNETIC FIELDS IN THE UNIVERSE: FROM LABORATORY AND STARS TO PRIMORDIAL STRUCTURES; v. 1311, p. 2-pg., 2010-01-01.

Resumo

This study report on the synthesis and characterization of molybdenum chlorophyllin (Mo-Chl) compounds associated in a complex with magnetic nanoparticles (citrate-coated cobalt ferrite), the latter prepared as a biocompatible magnetic fluid (MF). The complex material was developed for application as a synergic drug for cancer treatment using Photodynamic Therapy (PDT) and Hyperthermia Chlorophyllin was obtained from alkaline extraction of Ilex paraguariensis following molybdenum insertion from hydrolysis with molybdate sodium. Fluorescence quantum yield (Phi(f)) of Mo-Chl/dimethyl-sulphoxide (DMSO) was lower than 0.1, with a lifetime of 5.0 ns, as obtained from time-correlated single-photon counting technique. The oxygen quantum yield of Mo-Chl was carried out using laser flash-photolysis studies in homogeneous medium saturated with O-2(g) (Phi(Delta) = 0.50). Cellular viability was also evaluated via the classical MTT assay using gingival fibroblasts cells as a biological model. Studies performed with the complex Mo-Chl(5.0 mu mol.L-1)/MF at different magnetic nanoparticle concentrations (ranging from 10(12) to 10(15) particle.mL(-1)) revealed a cellular viability of approximately 95% for the ideal magnetic material concentration of 1 x 10(13) particle.mL(-1). The present study shows that natural photosensitizers molecules Mo-Chl used in association with magnetic nanoparticles represent a promising generation of drug developed to work synergistically in the treatment of neoplastic tissues using PDT and HPT. (AU)

• Processo FAPESP: 09/15363-6 - Desenvolvimento e caracterização de Equivalentes Dérmicos modificados com células tronco mesenquimais como modelos para avaliação de matriz extracelular após fotoestímulo com diodo-laser de baixa potência
• Beneficiário: Fernando Lucas Primo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/53719-4 - Utilização de nanocarreadores contendo fármacos fotossensíveis e outros ativos derivados de fatores de crescimento aplicados a processos fotodinâmicos nas áreas da saúde
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático