Salmonella enterica Typhimurium: establishment of cultivation condition on shake flask and flagellin isolation strategy by using tangencial ultrafiltration

Autor(es)

Oliveira, B. H. ; Silva, M. R. ; Braga, C. J. M. ; Massis, L. M. ; Ferreira, L. C. S. ; Takagi, M. E. Sbrogio-Almeida M. ; MendezVilas, A

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS IN INDUSTRY AND ENVIRONMENT: FROM SCIENTIFIC AND INDUSTRIAL RESEARCH TO CONSUMER PRODUCTS; v. N/A, p. 4-pg., 2011-01-01.

Resumo

Flagellin is a main protein present in the bacterial flagellum and its molecular mass is around 51.5 kDa. Its role as an adjuvant vaccine has been intensively studied and currently there is evidence to be an immunomodulador in situations of autoimmune diseases, co-administered with chemotherapy or as radioprotetor. This study focus on to establish the native flagellin production by Salmonella Typhimurium in shake flask and flagellin purification strategy by using tangencial ultrafiltration.. Results showed that flagellin can be released to the supernatant depend on the agitation speed, and tangencial ultrafiltration can be used as tool to purify this protein. (AU)

• Processo FAPESP: 06/52064-9 - Flagelinas de salmonella como adjuvantes vacinais administrados por vias de mucosa e parenteral.
• Beneficiário: Maria Elisabete Sbrogio-Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Regular